Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF675 and GFF676 are separated by 489 nucleotides GFF676 and GFF677 are separated by 91 nucleotides
GFF675 - Excinuclease ABC subunit B, at 31,497 to 33,518
GFF675
GFF676 - invasion plasmid antigen / internalin, putative, at 34,008 to 36,305
GFF676
GFF677 - Putative structural protein, at 36,397 to 37,305
GFF677
Position (kb)
34
35
36
37 Strain fitness (log2 ratio)
-1
0
1 at 33.069 kb on - strand, within GFF675 at 33.128 kb on + strand, within GFF675 at 33.228 kb on + strand, within GFF675 at 33.229 kb on - strand, within GFF675 at 33.549 kb on - strand at 33.581 kb on + strand at 33.591 kb on + strand at 33.592 kb on - strand at 33.773 kb on - strand at 33.776 kb on + strand at 33.796 kb on + strand at 33.796 kb on + strand at 33.804 kb on + strand at 33.805 kb on - strand at 33.840 kb on - strand at 34.013 kb on + strand at 34.222 kb on + strand at 34.231 kb on + strand at 34.257 kb on - strand, within GFF676 at 34.349 kb on + strand, within GFF676 at 34.567 kb on + strand, within GFF676 at 34.584 kb on - strand, within GFF676 at 34.594 kb on - strand, within GFF676 at 34.926 kb on - strand, within GFF676 at 35.065 kb on + strand, within GFF676 at 35.113 kb on + strand, within GFF676 at 35.259 kb on - strand, within GFF676 at 35.309 kb on + strand, within GFF676 at 35.309 kb on + strand, within GFF676 at 35.512 kb on - strand, within GFF676 at 35.599 kb on + strand, within GFF676 at 35.667 kb on + strand, within GFF676 at 36.018 kb on - strand, within GFF676 at 36.165 kb on - strand at 36.165 kb on - strand at 36.255 kb on - strand at 36.399 kb on - strand at 36.399 kb on - strand at 36.432 kb on + strand at 36.437 kb on - strand at 36.439 kb on + strand at 36.568 kb on - strand, within GFF677 at 36.569 kb on + strand, within GFF677 at 36.569 kb on + strand, within GFF677 at 36.570 kb on - strand, within GFF677 at 36.570 kb on - strand, within GFF677 at 37.185 kb on + strand, within GFF677 at 37.213 kb on + strand, within GFF677 at 37.214 kb on - strand, within GFF677 at 37.214 kb on - strand, within GFF677 at 37.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 33,069 - GFF675 0.78 +0.5 33,128 + GFF675 0.81 +0.3 33,228 + GFF675 0.86 -0.5 33,229 - GFF675 0.86 +0.6 33,549 - +0.2 33,581 + +1.3 33,591 + -0.3 33,592 - +0.1 33,773 - +0.3 33,776 + +0.5 33,796 + +0.2 33,796 + -0.1 33,804 + -0.3 33,805 - -0.4 33,840 - +1.1 34,013 + +0.0 34,222 + +0.2 34,231 + -0.1 34,257 - GFF676 0.11 +0.1 34,349 + GFF676 0.15 -0.3 34,567 + GFF676 0.24 -0.0 34,584 - GFF676 0.25 +0.3 34,594 - GFF676 0.26 -0.0 34,926 - GFF676 0.40 -0.1 35,065 + GFF676 0.46 -0.5 35,113 + GFF676 0.48 -1.4 35,259 - GFF676 0.54 -1.3 35,309 + GFF676 0.57 +0.1 35,309 + GFF676 0.57 -0.8 35,512 - GFF676 0.65 +0.2 35,599 + GFF676 0.69 +0.5 35,667 + GFF676 0.72 +0.2 36,018 - GFF676 0.87 +0.3 36,165 - -0.8 36,165 - +0.5 36,255 - +0.2 36,399 - -0.4 36,399 - +0.4 36,432 + -0.3 36,437 - +0.0 36,439 + +0.1 36,568 - GFF677 0.19 +0.5 36,569 + GFF677 0.19 +0.6 36,569 + GFF677 0.19 -0.9 36,570 - GFF677 0.19 -0.5 36,570 - GFF677 0.19 +0.0 37,185 + GFF677 0.87 -0.1 37,213 + GFF677 0.90 +1.5 37,214 - GFF677 0.90 -0.7 37,214 - GFF677 0.90 -0.7 37,244 - +0.2
Or see this region's nucleotide sequence