Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF61 and GFF62 are separated by 84 nucleotides GFF62 and GFF63 are separated by 4 nucleotides GFF63 and GFF64 are separated by 36 nucleotides GFF64 and GFF65 are separated by 100 nucleotides
GFF61 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), subgroup 1, at 44,727 to 46,244
GFF61
GFF62 - '5'-methylthioadenosine nucleosidase (EC 3.2.2.16) / S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)' transl_table=11, at 46,329 to 47,027
GFF62
GFF63 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 47,032 to 47,820
GFF63
GFF64 - Putative inner membrane protein, at 47,857 to 48,480
GFF64
GFF65 - ErpA, essential respiratory protein A / probable iron binding protein from the HesB_IscA_SufA family, at 48,581 to 48,925
GFF65
Position (kb) 47
48 Strain fitness (log2 ratio) -2
-1
0
1 at 46.053 kb on - strand, within GFF61 at 46.053 kb on - strand, within GFF61 at 46.111 kb on - strand at 46.113 kb on - strand at 46.127 kb on + strand at 46.294 kb on + strand at 47.318 kb on + strand, within GFF63 at 47.318 kb on + strand, within GFF63 at 47.326 kb on - strand, within GFF63 at 47.467 kb on + strand, within GFF63 at 47.524 kb on - strand, within GFF63 at 47.532 kb on + strand, within GFF63 at 47.533 kb on - strand, within GFF63 at 47.699 kb on - strand, within GFF63 at 47.796 kb on + strand at 47.797 kb on - strand at 47.883 kb on - strand at 47.895 kb on - strand at 48.072 kb on + strand, within GFF64 at 48.072 kb on + strand, within GFF64 at 48.178 kb on - strand, within GFF64 at 48.206 kb on + strand, within GFF64 at 48.305 kb on - strand, within GFF64 at 48.319 kb on - strand, within GFF64 at 48.319 kb on - strand, within GFF64 at 48.354 kb on + strand, within GFF64 at 48.354 kb on + strand, within GFF64 at 48.481 kb on + strand at 48.481 kb on + strand at 48.534 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 46,053 - GFF61 0.87 +0.4 46,053 - GFF61 0.87 -1.8 46,111 - +0.2 46,113 - -0.8 46,127 + -0.3 46,294 + +0.5 47,318 + GFF63 0.36 -0.1 47,318 + GFF63 0.36 -0.4 47,326 - GFF63 0.37 -0.4 47,467 + GFF63 0.55 -0.7 47,524 - GFF63 0.62 -0.4 47,532 + GFF63 0.63 -0.0 47,533 - GFF63 0.63 -0.6 47,699 - GFF63 0.85 -0.3 47,796 + +0.7 47,797 - -0.3 47,883 - +0.7 47,895 - +0.0 48,072 + GFF64 0.34 +1.1 48,072 + GFF64 0.34 -0.5 48,178 - GFF64 0.51 -0.3 48,206 + GFF64 0.56 +0.4 48,305 - GFF64 0.72 +0.5 48,319 - GFF64 0.74 -1.5 48,319 - GFF64 0.74 +0.0 48,354 + GFF64 0.80 -0.3 48,354 + GFF64 0.80 +0.1 48,481 + -0.8 48,481 + -1.1 48,534 - +0.7
Or see this region's nucleotide sequence