Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF611 and GFF612 are separated by 98 nucleotides GFF612 and GFF613 are separated by 225 nucleotides GFF613 and GFF614 are separated by 5 nucleotides
GFF611 - Transcription accessory protein (S1 RNA-binding domain), at 349,626 to 351,956
GFF611
GFF612 - Transcription elongation factor GreB, at 352,055 to 352,528
GFF612
GFF613 - Two-component system response regulator OmpR, at 352,754 to 353,473
GFF613
GFF614 - Osmolarity sensory histidine kinase EnvZ, at 353,479 to 354,822
GFF614
Position (kb) 352
353 Strain fitness (log2 ratio) -2
-1
0
1
2 at 351.199 kb on + strand, within GFF611 at 351.200 kb on - strand, within GFF611 at 351.308 kb on + strand, within GFF611 at 351.409 kb on + strand, within GFF611 at 351.410 kb on - strand, within GFF611 at 351.417 kb on + strand, within GFF611 at 351.669 kb on - strand, within GFF611 at 351.929 kb on + strand at 351.961 kb on + strand at 351.962 kb on - strand at 352.066 kb on - strand at 352.186 kb on + strand, within GFF612 at 352.187 kb on - strand, within GFF612 at 352.375 kb on + strand, within GFF612 at 352.379 kb on - strand, within GFF612 at 352.381 kb on - strand, within GFF612 at 352.470 kb on + strand, within GFF612 at 352.471 kb on - strand, within GFF612 at 352.540 kb on - strand at 352.571 kb on - strand at 352.572 kb on + strand at 352.599 kb on - strand at 352.713 kb on + strand at 352.714 kb on - strand at 352.721 kb on + strand at 352.721 kb on + strand at 352.721 kb on + strand at 352.747 kb on - strand at 352.792 kb on + strand at 352.912 kb on - strand, within GFF613 at 352.912 kb on - strand, within GFF613 at 352.921 kb on - strand, within GFF613 at 352.993 kb on + strand, within GFF613 at 353.057 kb on + strand, within GFF613 at 353.112 kb on + strand, within GFF613 at 353.303 kb on - strand, within GFF613 at 353.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 351,199 + GFF611 0.67 +0.7 351,200 - GFF611 0.68 +0.1 351,308 + GFF611 0.72 +1.0 351,409 + GFF611 0.76 +0.4 351,410 - GFF611 0.77 -0.2 351,417 + GFF611 0.77 +2.1 351,669 - GFF611 0.88 -0.2 351,929 + -0.4 351,961 + -0.1 351,962 - +0.5 352,066 - +0.1 352,186 + GFF612 0.28 -0.4 352,187 - GFF612 0.28 -0.5 352,375 + GFF612 0.68 -0.1 352,379 - GFF612 0.68 -0.9 352,381 - GFF612 0.69 +0.5 352,470 + GFF612 0.88 -2.0 352,471 - GFF612 0.88 -0.1 352,540 - +0.4 352,571 - +0.0 352,572 + +0.5 352,599 - +1.2 352,713 + -0.8 352,714 - +0.2 352,721 + +0.4 352,721 + +0.4 352,721 + -0.3 352,747 - +0.1 352,792 + +0.1 352,912 - GFF613 0.22 +0.7 352,912 - GFF613 0.22 +1.6 352,921 - GFF613 0.23 +0.2 352,993 + GFF613 0.33 +0.4 353,057 + GFF613 0.42 +1.5 353,112 + GFF613 0.50 -0.7 353,303 - GFF613 0.76 -0.0 353,417 - +1.1
Or see this region's nucleotide sequence