Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4618 and GFF4619 are separated by 323 nucleotides GFF4619 and GFF4620 are separated by 59 nucleotides GFF4620 and GFF4621 are separated by 648 nucleotides GFF4621 and GFF4622 are separated by 43 nucleotides
GFF4618 - Lactam utilization protein LamB, at 410,923 to 411,657
GFF4618
GFF4619 - hypothetical protein, at 411,981 to 412,112
GFF4619
GFF4620 - Probable DNA recombinase, at 412,172 to 412,738
GFF4620
GFF4621 - putative inner membrane protein, at 413,387 to 413,533
GFF4621
GFF4622 - FIG074102: hypothetical protein, at 413,577 to 414,275
GFF4622
Position (kb) 412
413 Strain fitness (log2 ratio) -1
0
1
2 at 411.250 kb on + strand, within GFF4618 at 411.251 kb on - strand, within GFF4618 at 411.310 kb on + strand, within GFF4618 at 411.311 kb on - strand, within GFF4618 at 411.429 kb on + strand, within GFF4618 at 411.506 kb on + strand, within GFF4618 at 411.506 kb on + strand, within GFF4618 at 411.506 kb on + strand, within GFF4618 at 411.507 kb on - strand, within GFF4618 at 411.507 kb on - strand, within GFF4618 at 411.681 kb on + strand at 411.821 kb on - strand at 411.859 kb on + strand at 412.063 kb on - strand, within GFF4619 at 412.088 kb on + strand, within GFF4619 at 412.350 kb on - strand, within GFF4620 at 412.350 kb on - strand, within GFF4620 at 412.406 kb on + strand, within GFF4620 at 412.406 kb on + strand, within GFF4620 at 412.409 kb on - strand, within GFF4620 at 412.443 kb on - strand, within GFF4620 at 412.577 kb on - strand, within GFF4620 at 412.577 kb on - strand, within GFF4620 at 412.625 kb on - strand, within GFF4620 at 412.635 kb on + strand, within GFF4620 at 412.636 kb on - strand, within GFF4620 at 412.636 kb on - strand, within GFF4620 at 412.643 kb on + strand, within GFF4620 at 412.690 kb on + strand at 412.743 kb on - strand at 413.175 kb on - strand at 413.434 kb on + strand, within GFF4621 at 413.434 kb on + strand, within GFF4621 at 413.554 kb on + strand at 413.711 kb on - strand, within GFF4622
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 411,250 + GFF4618 0.44 +0.6 411,251 - GFF4618 0.45 -0.1 411,310 + GFF4618 0.53 -0.1 411,311 - GFF4618 0.53 +0.7 411,429 + GFF4618 0.69 +0.1 411,506 + GFF4618 0.79 -0.7 411,506 + GFF4618 0.79 +0.3 411,506 + GFF4618 0.79 -0.7 411,507 - GFF4618 0.79 +0.3 411,507 - GFF4618 0.79 +0.9 411,681 + -0.1 411,821 - +0.3 411,859 + +0.0 412,063 - GFF4619 0.62 -0.7 412,088 + GFF4619 0.81 +1.9 412,350 - GFF4620 0.31 -0.6 412,350 - GFF4620 0.31 -0.1 412,406 + GFF4620 0.41 -0.1 412,406 + GFF4620 0.41 -0.9 412,409 - GFF4620 0.42 +0.5 412,443 - GFF4620 0.48 -0.1 412,577 - GFF4620 0.71 +0.2 412,577 - GFF4620 0.71 -0.5 412,625 - GFF4620 0.80 +0.0 412,635 + GFF4620 0.82 +0.3 412,636 - GFF4620 0.82 -0.5 412,636 - GFF4620 0.82 -0.5 412,643 + GFF4620 0.83 -0.2 412,690 + +1.1 412,743 - +2.3 413,175 - +0.3 413,434 + GFF4621 0.32 +0.2 413,434 + GFF4621 0.32 +0.6 413,554 + +0.3 413,711 - GFF4622 0.19 +1.0
Or see this region's nucleotide sequence