Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4564 and GFF4565 are separated by 11 nucleotides GFF4565 and GFF4566 overlap by 4 nucleotides
GFF4564 - Putative molecular chaperone, DnaJ family, at 349,116 to 350,549
GFF4564
GFF4565 - FIG00637867: hypothetical protein, at 350,561 to 351,256
GFF4565
GFF4566 - FIG00638911: hypothetical protein, at 351,253 to 352,725
GFF4566
Position (kb) 350
351
352 Strain fitness (log2 ratio) -1
0
1
2 at 349.763 kb on - strand, within GFF4564 at 349.881 kb on + strand, within GFF4564 at 349.881 kb on + strand, within GFF4564 at 349.882 kb on - strand, within GFF4564 at 349.882 kb on - strand, within GFF4564 at 349.884 kb on + strand, within GFF4564 at 350.085 kb on + strand, within GFF4564 at 350.085 kb on + strand, within GFF4564 at 350.085 kb on + strand, within GFF4564 at 350.086 kb on - strand, within GFF4564 at 350.232 kb on + strand, within GFF4564 at 350.245 kb on - strand, within GFF4564 at 350.258 kb on + strand, within GFF4564 at 350.527 kb on + strand at 350.528 kb on - strand at 350.531 kb on - strand at 350.616 kb on + strand at 350.616 kb on + strand at 350.617 kb on - strand at 350.617 kb on - strand at 350.620 kb on + strand at 350.620 kb on + strand at 350.642 kb on - strand, within GFF4565 at 350.657 kb on + strand, within GFF4565 at 350.658 kb on - strand, within GFF4565 at 350.658 kb on - strand, within GFF4565 at 350.660 kb on - strand, within GFF4565 at 350.732 kb on + strand, within GFF4565 at 350.733 kb on - strand, within GFF4565 at 350.791 kb on + strand, within GFF4565 at 350.797 kb on - strand, within GFF4565 at 350.915 kb on + strand, within GFF4565 at 351.002 kb on + strand, within GFF4565 at 351.018 kb on - strand, within GFF4565 at 351.041 kb on + strand, within GFF4565 at 351.097 kb on - strand, within GFF4565 at 351.190 kb on - strand at 351.190 kb on - strand at 351.235 kb on + strand at 351.258 kb on + strand at 351.306 kb on + strand at 351.465 kb on - strand, within GFF4566 at 351.574 kb on - strand, within GFF4566 at 351.891 kb on + strand, within GFF4566 at 351.906 kb on + strand, within GFF4566 at 352.009 kb on + strand, within GFF4566 at 352.010 kb on - strand, within GFF4566 at 352.010 kb on - strand, within GFF4566 at 352.010 kb on - strand, within GFF4566 at 352.012 kb on + strand, within GFF4566 at 352.202 kb on - strand, within GFF4566 at 352.202 kb on - strand, within GFF4566
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 349,763 - GFF4564 0.45 +0.8 349,881 + GFF4564 0.53 +0.2 349,881 + GFF4564 0.53 -0.6 349,882 - GFF4564 0.53 +0.1 349,882 - GFF4564 0.53 +0.3 349,884 + GFF4564 0.54 -1.5 350,085 + GFF4564 0.68 +0.0 350,085 + GFF4564 0.68 +0.1 350,085 + GFF4564 0.68 -0.8 350,086 - GFF4564 0.68 +1.5 350,232 + GFF4564 0.78 -0.1 350,245 - GFF4564 0.79 +0.8 350,258 + GFF4564 0.80 -1.1 350,527 + +0.5 350,528 - +0.4 350,531 - -0.0 350,616 + +0.2 350,616 + -0.1 350,617 - -0.1 350,617 - +0.2 350,620 + +0.9 350,620 + +0.1 350,642 - GFF4565 0.12 +1.5 350,657 + GFF4565 0.14 +0.5 350,658 - GFF4565 0.14 +0.6 350,658 - GFF4565 0.14 +2.2 350,660 - GFF4565 0.14 +0.5 350,732 + GFF4565 0.25 -0.9 350,733 - GFF4565 0.25 +0.4 350,791 + GFF4565 0.33 +0.4 350,797 - GFF4565 0.34 +0.1 350,915 + GFF4565 0.51 -0.3 351,002 + GFF4565 0.63 +0.5 351,018 - GFF4565 0.66 -0.7 351,041 + GFF4565 0.69 +0.8 351,097 - GFF4565 0.77 +1.7 351,190 - +0.1 351,190 - +0.6 351,235 + -0.1 351,258 + +0.0 351,306 + -0.0 351,465 - GFF4566 0.14 -0.3 351,574 - GFF4566 0.22 -0.7 351,891 + GFF4566 0.43 +0.7 351,906 + GFF4566 0.44 +0.8 352,009 + GFF4566 0.51 -0.5 352,010 - GFF4566 0.51 +1.5 352,010 - GFF4566 0.51 -0.1 352,010 - GFF4566 0.51 -1.3 352,012 + GFF4566 0.52 -0.1 352,202 - GFF4566 0.64 +0.3 352,202 - GFF4566 0.64 -0.1
Or see this region's nucleotide sequence