Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4368 and GFF4369 are separated by 99 nucleotides GFF4369 and GFF4370 are separated by 214 nucleotides GFF4370 and GFF4371 are separated by 32 nucleotides
GFF4368 - Ammonium transporter, at 151,108 to 152,394
GFF4368
GFF4369 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 152,494 to 153,354
GFF4369
GFF4370 - Glycoprotein-polysaccharide metabolism, at 153,569 to 154,138
GFF4370
GFF4371 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 154,171 to 154,560
GFF4371
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1
2 at 151.592 kb on - strand, within GFF4368 at 151.592 kb on - strand, within GFF4368 at 151.592 kb on - strand, within GFF4368 at 151.666 kb on - strand, within GFF4368 at 151.716 kb on - strand, within GFF4368 at 151.716 kb on - strand, within GFF4368 at 151.929 kb on - strand, within GFF4368 at 151.929 kb on - strand, within GFF4368 at 152.005 kb on + strand, within GFF4368 at 152.079 kb on + strand, within GFF4368 at 152.080 kb on - strand, within GFF4368 at 152.114 kb on + strand, within GFF4368 at 152.248 kb on - strand, within GFF4368 at 152.254 kb on - strand, within GFF4368 at 152.384 kb on - strand at 152.386 kb on - strand at 152.424 kb on + strand at 152.439 kb on - strand at 152.559 kb on - strand at 152.614 kb on - strand, within GFF4369 at 152.615 kb on + strand, within GFF4369 at 152.616 kb on - strand, within GFF4369 at 152.616 kb on - strand, within GFF4369 at 152.616 kb on - strand, within GFF4369 at 152.658 kb on + strand, within GFF4369 at 152.658 kb on + strand, within GFF4369 at 152.659 kb on - strand, within GFF4369 at 152.659 kb on - strand, within GFF4369 at 152.659 kb on - strand, within GFF4369 at 153.100 kb on - strand, within GFF4369 at 153.354 kb on + strand at 153.354 kb on + strand at 153.355 kb on - strand at 153.358 kb on + strand at 153.401 kb on + strand at 153.402 kb on - strand at 153.685 kb on + strand, within GFF4370 at 153.685 kb on + strand, within GFF4370 at 153.698 kb on - strand, within GFF4370 at 153.835 kb on - strand, within GFF4370 at 153.893 kb on - strand, within GFF4370 at 153.956 kb on - strand, within GFF4370 at 153.997 kb on + strand, within GFF4370 at 154.172 kb on + strand at 154.275 kb on + strand, within GFF4371
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 151,592 - GFF4368 0.38 -0.3 151,592 - GFF4368 0.38 -0.6 151,592 - GFF4368 0.38 +0.7 151,666 - GFF4368 0.43 +0.1 151,716 - GFF4368 0.47 +0.1 151,716 - GFF4368 0.47 +0.0 151,929 - GFF4368 0.64 -0.2 151,929 - GFF4368 0.64 -0.7 152,005 + GFF4368 0.70 +1.1 152,079 + GFF4368 0.75 +0.5 152,080 - GFF4368 0.76 +1.9 152,114 + GFF4368 0.78 +0.1 152,248 - GFF4368 0.89 -2.1 152,254 - GFF4368 0.89 +0.2 152,384 - +0.8 152,386 - -0.1 152,424 + -0.4 152,439 - +0.1 152,559 - -0.3 152,614 - GFF4369 0.14 -0.8 152,615 + GFF4369 0.14 -0.1 152,616 - GFF4369 0.14 -0.4 152,616 - GFF4369 0.14 -0.9 152,616 - GFF4369 0.14 -0.1 152,658 + GFF4369 0.19 +0.3 152,658 + GFF4369 0.19 -1.3 152,659 - GFF4369 0.19 -0.6 152,659 - GFF4369 0.19 +0.9 152,659 - GFF4369 0.19 -0.7 153,100 - GFF4369 0.70 -0.2 153,354 + +0.0 153,354 + +0.8 153,355 - +0.3 153,358 + +0.2 153,401 + -1.0 153,402 - -0.1 153,685 + GFF4370 0.20 +0.1 153,685 + GFF4370 0.20 +0.2 153,698 - GFF4370 0.23 +0.2 153,835 - GFF4370 0.47 -0.2 153,893 - GFF4370 0.57 -0.2 153,956 - GFF4370 0.68 -0.6 153,997 + GFF4370 0.75 -0.1 154,172 + -0.1 154,275 + GFF4371 0.27 -0.1
Or see this region's nucleotide sequence