Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3960 and GFF3961 overlap by 4 nucleotides GFF3961 and GFF3962 overlap by 4 nucleotides GFF3962 and GFF3963 are separated by 1 nucleotides GFF3963 and GFF3964 overlap by 14 nucleotides GFF3964 and GFF3965 are separated by 8 nucleotides
GFF3960 - Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3), at 239,645 to 240,439
GFF3960
GFF3961 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 240,436 to 241,149
GFF3961
GFF3962 - Substrate-specific component CbiM of cobalt ECF transporter, at 241,146 to 241,883
GFF3962
GFF3963 - Additional substrate-specific component CbiN of cobalt ECF transporter, at 241,885 to 242,166
GFF3963
GFF3964 - Transmembrane component CbiQ of energizing module of cobalt ECF transporter, at 242,153 to 242,830
GFF3964
GFF3965 - ATPase component CbiO of energizing module of cobalt ECF transporter, at 242,839 to 243,654
GFF3965
Position (kb) 241
242 Strain fitness (log2 ratio) -2
-1
0
1 at 240.459 kb on - strand at 240.611 kb on + strand, within GFF3961 at 240.612 kb on - strand, within GFF3961 at 240.724 kb on - strand, within GFF3961 at 240.864 kb on - strand, within GFF3961 at 240.927 kb on - strand, within GFF3961 at 241.125 kb on + strand at 241.126 kb on - strand at 241.139 kb on - strand at 241.288 kb on + strand, within GFF3962 at 241.297 kb on + strand, within GFF3962 at 241.466 kb on + strand, within GFF3962 at 242.019 kb on + strand, within GFF3963 at 242.019 kb on + strand, within GFF3963 at 242.020 kb on - strand, within GFF3963 at 242.020 kb on - strand, within GFF3963 at 242.039 kb on + strand, within GFF3963 at 242.241 kb on + strand, within GFF3964 at 242.323 kb on - strand, within GFF3964 at 242.393 kb on - strand, within GFF3964 at 242.436 kb on + strand, within GFF3964 at 242.551 kb on - strand, within GFF3964 at 242.828 kb on + strand at 242.835 kb on + strand at 242.838 kb on + strand at 242.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 240,459 - -0.8 240,611 + GFF3961 0.25 +0.3 240,612 - GFF3961 0.25 +0.1 240,724 - GFF3961 0.40 -0.1 240,864 - GFF3961 0.60 -0.1 240,927 - GFF3961 0.69 -1.2 241,125 + +0.8 241,126 - +0.5 241,139 - -0.0 241,288 + GFF3962 0.19 +0.8 241,297 + GFF3962 0.20 -0.1 241,466 + GFF3962 0.43 -1.8 242,019 + GFF3963 0.48 -1.6 242,019 + GFF3963 0.48 -0.3 242,020 - GFF3963 0.48 +0.5 242,020 - GFF3963 0.48 -0.5 242,039 + GFF3963 0.55 -0.6 242,241 + GFF3964 0.13 +0.3 242,323 - GFF3964 0.25 -0.8 242,393 - GFF3964 0.35 -0.6 242,436 + GFF3964 0.42 -0.4 242,551 - GFF3964 0.59 -0.1 242,828 + -0.3 242,835 + -0.8 242,838 + +0.3 242,839 - -0.8
Or see this region's nucleotide sequence