Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3950 and GFF3951 overlap by 4 nucleotides GFF3951 and GFF3952 are separated by 10 nucleotides GFF3952 and GFF3953 overlap by 1 nucleotides
GFF3950 - Cobyrinic acid A,C-diamide synthase, at 231,052 to 232,431
GFF3950
GFF3951 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 232,428 to 233,387
GFF3951
GFF3952 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 233,398 to 234,030
GFF3952
GFF3953 - Cobalt-precorrin-6 synthase, anaerobic, at 234,030 to 235,169
GFF3953
Position (kb) 232
233
234 Strain fitness (log2 ratio) -2
-1
0
1
2 at 231.590 kb on - strand, within GFF3950 at 231.834 kb on + strand, within GFF3950 at 231.841 kb on + strand, within GFF3950 at 231.965 kb on + strand, within GFF3950 at 231.968 kb on - strand, within GFF3950 at 232.066 kb on + strand, within GFF3950 at 232.169 kb on - strand, within GFF3950 at 232.244 kb on - strand, within GFF3950 at 232.287 kb on + strand, within GFF3950 at 232.427 kb on + strand at 232.594 kb on + strand, within GFF3951 at 233.046 kb on + strand, within GFF3951 at 233.145 kb on + strand, within GFF3951 at 233.148 kb on - strand, within GFF3951 at 233.156 kb on + strand, within GFF3951 at 233.225 kb on + strand, within GFF3951 at 233.225 kb on + strand, within GFF3951 at 233.397 kb on + strand at 233.398 kb on - strand at 233.398 kb on - strand at 233.398 kb on - strand at 233.404 kb on + strand at 233.404 kb on + strand at 233.405 kb on - strand at 233.407 kb on - strand at 233.448 kb on + strand at 233.623 kb on - strand, within GFF3952 at 233.703 kb on + strand, within GFF3952 at 233.703 kb on + strand, within GFF3952 at 233.703 kb on + strand, within GFF3952 at 233.704 kb on - strand, within GFF3952 at 233.704 kb on - strand, within GFF3952 at 233.778 kb on + strand, within GFF3952 at 233.853 kb on + strand, within GFF3952 at 234.011 kb on - strand at 234.090 kb on + strand at 234.090 kb on + strand at 234.091 kb on - strand at 234.092 kb on + strand at 234.092 kb on + strand at 234.143 kb on - strand at 234.146 kb on - strand, within GFF3953 at 234.200 kb on - strand, within GFF3953
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 231,590 - GFF3950 0.39 +0.0 231,834 + GFF3950 0.57 +0.7 231,841 + GFF3950 0.57 -1.1 231,965 + GFF3950 0.66 +0.4 231,968 - GFF3950 0.66 -0.1 232,066 + GFF3950 0.73 -0.3 232,169 - GFF3950 0.81 -0.1 232,244 - GFF3950 0.86 +0.3 232,287 + GFF3950 0.89 +0.1 232,427 + -0.4 232,594 + GFF3951 0.17 +0.2 233,046 + GFF3951 0.64 +0.2 233,145 + GFF3951 0.75 -0.6 233,148 - GFF3951 0.75 -0.4 233,156 + GFF3951 0.76 -0.5 233,225 + GFF3951 0.83 +1.8 233,225 + GFF3951 0.83 -0.8 233,397 + +0.2 233,398 - -0.1 233,398 - +0.8 233,398 - +0.7 233,404 + +0.1 233,404 + +0.4 233,405 - +0.8 233,407 - -0.6 233,448 + +0.1 233,623 - GFF3952 0.36 -0.7 233,703 + GFF3952 0.48 +1.0 233,703 + GFF3952 0.48 -0.3 233,703 + GFF3952 0.48 +0.1 233,704 - GFF3952 0.48 +0.7 233,704 - GFF3952 0.48 +0.8 233,778 + GFF3952 0.60 -0.1 233,853 + GFF3952 0.72 +2.2 234,011 - -0.2 234,090 + +0.6 234,090 + +0.0 234,091 - +0.6 234,092 + +1.5 234,092 + -2.0 234,143 - -0.2 234,146 - GFF3953 0.10 +0.5 234,200 - GFF3953 0.15 +2.6
Or see this region's nucleotide sequence