Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF388 and GFF389 are separated by 56 nucleotides GFF389 and GFF390 are separated by 39 nucleotides GFF390 and GFF391 overlap by 1 nucleotides
GFF388 - Lipopolysaccharide 1,2-N-acetylglucosaminetransferase (EC 2.4.1.56), at 102,372 to 103,517
GFF388
GFF389 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 103,574 to 104,788
GFF389
GFF390 - Lipopolysaccharide heptosyltransferase I (EC 2.4.1.-), at 104,828 to 105,781
GFF390
GFF391 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 105,781 to 106,827
GFF391
Position (kb) 103
104
105 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 102.641 kb on + strand, within GFF388 at 102.671 kb on + strand, within GFF388 at 102.671 kb on + strand, within GFF388 at 102.690 kb on - strand, within GFF388 at 102.759 kb on - strand, within GFF388 at 103.029 kb on - strand, within GFF388 at 103.148 kb on + strand, within GFF388 at 103.152 kb on - strand, within GFF388 at 103.196 kb on + strand, within GFF388 at 103.198 kb on + strand, within GFF388 at 103.355 kb on + strand, within GFF388 at 103.373 kb on - strand, within GFF388 at 103.553 kb on - strand at 103.555 kb on + strand at 103.764 kb on - strand, within GFF389 at 103.882 kb on + strand, within GFF389 at 104.016 kb on - strand, within GFF389 at 104.144 kb on - strand, within GFF389 at 104.197 kb on - strand, within GFF389 at 104.947 kb on - strand, within GFF390 at 104.949 kb on - strand, within GFF390 at 104.956 kb on + strand, within GFF390 at 105.243 kb on + strand, within GFF390 at 105.322 kb on - strand, within GFF390 at 105.740 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 102,641 + GFF388 0.23 -5.3 102,671 + GFF388 0.26 -2.6 102,671 + GFF388 0.26 -4.3 102,690 - GFF388 0.28 -1.7 102,759 - GFF388 0.34 -0.3 103,029 - GFF388 0.57 -6.5 103,148 + GFF388 0.68 -4.7 103,152 - GFF388 0.68 -2.3 103,196 + GFF388 0.72 -5.5 103,198 + GFF388 0.72 -2.1 103,355 + GFF388 0.86 -4.1 103,373 - GFF388 0.87 -5.7 103,553 - -0.5 103,555 + +0.5 103,764 - GFF389 0.16 -1.7 103,882 + GFF389 0.25 -2.6 104,016 - GFF389 0.36 -3.2 104,144 - GFF389 0.47 -1.4 104,197 - GFF389 0.51 -1.3 104,947 - GFF390 0.12 +0.4 104,949 - GFF390 0.13 +0.7 104,956 + GFF390 0.13 -0.2 105,243 + GFF390 0.44 -0.2 105,322 - GFF390 0.52 -2.3 105,740 + -2.1
Or see this region's nucleotide sequence