Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF366 and GFF367 are separated by 78 nucleotides GFF367 and GFF368 are separated by 77 nucleotides GFF368 and GFF369 are separated by 107 nucleotides GFF369 and GFF370 overlap by 20 nucleotides
GFF366 - Ribonuclease PH (EC 2.7.7.56), at 85,683 to 86,399
GFF366
GFF367 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 86,478 to 87,119
GFF367
GFF368 - Transcriptional regulator SlmA, TetR family, at 87,197 to 87,793
GFF368
GFF369 - 'Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23)' transl_table=11, at 87,901 to 88,356
GFF369
GFF370 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 88,337 to 89,560
GFF370
Position (kb)
87
88 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 86.282 kb on - strand, within GFF366 at 86.488 kb on - strand at 86.546 kb on + strand, within GFF367 at 86.598 kb on - strand, within GFF367 at 86.825 kb on - strand, within GFF367 at 86.868 kb on - strand, within GFF367 at 86.894 kb on + strand, within GFF367 at 86.997 kb on + strand, within GFF367 at 87.005 kb on + strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.006 kb on - strand, within GFF367 at 87.151 kb on - strand at 87.151 kb on - strand at 87.290 kb on - strand, within GFF368 at 87.343 kb on - strand, within GFF368 at 87.375 kb on - strand, within GFF368 at 87.844 kb on - strand at 87.875 kb on + strand at 87.875 kb on + strand at 87.902 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 86,282 - GFF366 0.84 +0.7 86,488 - -2.0 86,546 + GFF367 0.11 +0.2 86,598 - GFF367 0.19 -0.6 86,825 - GFF367 0.54 -0.1 86,868 - GFF367 0.61 +2.3 86,894 + GFF367 0.65 -0.1 86,997 + GFF367 0.81 -0.1 87,005 + GFF367 0.82 +0.3 87,006 - GFF367 0.82 +0.6 87,006 - GFF367 0.82 +1.0 87,006 - GFF367 0.82 -0.2 87,006 - GFF367 0.82 -0.7 87,006 - GFF367 0.82 +0.2 87,151 - -0.2 87,151 - -0.2 87,290 - GFF368 0.16 +0.2 87,343 - GFF368 0.24 -1.1 87,375 - GFF368 0.30 -0.9 87,844 - +0.1 87,875 + +0.1 87,875 + -0.2 87,902 + +2.6
Or see this region's nucleotide sequence