Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF360

Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF358 and GFF359 are separated by 54 nucleotidesGFF359 and GFF360 are separated by 64 nucleotidesGFF360 and GFF361 are separated by 42 nucleotides GFF358 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 79,027 to 79,302 GFF358 GFF359 - Guanylate kinase (EC 2.7.4.8), at 79,357 to 79,980 GFF359 GFF360 - hypothetical protein, at 80,045 to 80,194 GFF360 GFF361 - DNA ligase (EC 6.5.1.2), LigB, at 80,237 to 81,922 GFF361 Position (kb) 80 81Strain fitness (log2 ratio) -2 -1 0 1at 79.064 kb on - strand, within GFF358at 79.303 kb on - strandat 79.307 kb on - strandat 79.414 kb on - strandat 80.029 kb on - strandat 80.029 kb on - strandat 80.029 kb on - strandat 80.033 kb on + strandat 80.056 kb on - strandat 80.175 kb on - strand, within GFF360at 80.195 kb on + strandat 80.254 kb on + strandat 80.542 kb on + strand, within GFF361at 80.565 kb on + strand, within GFF361at 80.565 kb on + strand, within GFF361at 80.628 kb on + strand, within GFF361at 80.728 kb on + strand, within GFF361at 80.728 kb on + strand, within GFF361at 80.729 kb on - strand, within GFF361at 80.769 kb on + strand, within GFF361at 80.788 kb on - strand, within GFF361at 80.803 kb on + strand, within GFF361at 80.803 kb on + strand, within GFF361at 80.804 kb on - strand, within GFF361at 80.902 kb on + strand, within GFF361at 80.946 kb on + strand, within GFF361at 80.987 kb on + strand, within GFF361at 80.987 kb on + strand, within GFF361at 80.989 kb on + strand, within GFF361at 81.075 kb on + strand, within GFF361at 81.082 kb on - strand, within GFF361at 81.082 kb on - strand, within GFF361at 81.152 kb on + strand, within GFF361

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI
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79,064 - GFF358 0.13 -2.7
79,303 - -0.1
79,307 - -0.3
79,414 - -0.0
80,029 - +0.8
80,029 - -0.3
80,029 - +0.3
80,033 + -0.4
80,056 - -0.3
80,175 - GFF360 0.87 -0.7
80,195 + -1.0
80,254 + +1.1
80,542 + GFF361 0.18 -0.2
80,565 + GFF361 0.19 +0.1
80,565 + GFF361 0.19 -0.3
80,628 + GFF361 0.23 +0.7
80,728 + GFF361 0.29 -0.5
80,728 + GFF361 0.29 -0.1
80,729 - GFF361 0.29 -0.4
80,769 + GFF361 0.32 -0.9
80,788 - GFF361 0.33 +0.1
80,803 + GFF361 0.34 -0.7
80,803 + GFF361 0.34 -0.0
80,804 - GFF361 0.34 +0.8
80,902 + GFF361 0.39 -0.1
80,946 + GFF361 0.42 -0.3
80,987 + GFF361 0.44 -0.4
80,987 + GFF361 0.44 -0.2
80,989 + GFF361 0.45 +0.5
81,075 + GFF361 0.50 +0.7
81,082 - GFF361 0.50 +0.1
81,082 - GFF361 0.50 -1.1
81,152 + GFF361 0.54 -0.1

Or see this region's nucleotide sequence