Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3599

Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3595 and GFF3596 are separated by 93 nucleotidesGFF3596 and GFF3597 overlap by 23 nucleotidesGFF3597 and GFF3598 overlap by 4 nucleotidesGFF3598 and GFF3599 are separated by 56 nucleotidesGFF3599 and GFF3600 are separated by 17 nucleotidesGFF3600 and GFF3601 overlap by 4 nucleotides GFF3595 - Secretion system effector SseG, at 350,909 to 351,496 GFF3595 GFF3596 - Type III secretion protein SsaG, at 351,590 to 351,805 GFF3596 GFF3597 - Type III secretion protein SsaH, at 351,783 to 352,073 GFF3597 GFF3598 - Type III secretion protein SsaI, at 352,070 to 352,333 GFF3598 GFF3599 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 352,390 to 353,079 GFF3599 GFF3600 - FIG029138: Type III secretion protein, at 353,097 to 353,645 GFF3600 GFF3601 - Type III secretion protein SsaK, at 353,642 to 354,316 GFF3601 Position (kb) 352 353 354Strain fitness (log2 ratio) -2 -1 0 1at 352.349 kb on + strandat 352.366 kb on - strandat 352.494 kb on - strand, within GFF3599at 352.608 kb on - strand, within GFF3599at 352.990 kb on + strand, within GFF3599at 353.467 kb on + strand, within GFF3600at 353.937 kb on + strand, within GFF3601at 354.003 kb on + strand, within GFF3601at 354.053 kb on - strand, within GFF3601

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI
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352,349 + -1.0
352,366 - +0.9
352,494 - GFF3599 0.15 -1.8
352,608 - GFF3599 0.32 -0.2
352,990 + GFF3599 0.87 -0.3
353,467 + GFF3600 0.67 +0.0
353,937 + GFF3601 0.44 +0.1
354,003 + GFF3601 0.53 -0.1
354,053 - GFF3601 0.61 +0.4

Or see this region's nucleotide sequence