Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF356

Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF355 and GFF356 are separated by 5 nucleotidesGFF356 and GFF357 are separated by 4 nucleotides GFF355 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 74,116 to 76,197 GFF355 GFF356 - 'tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)' transl_table=11, at 76,203 to 76,892 GFF356 GFF357 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 76,897 to 79,008 GFF357 Position (kb) 76 77Strain fitness (log2 ratio) -1 0 1 2 3at 75.430 kb on + strand, within GFF355at 75.699 kb on + strand, within GFF355at 76.128 kb on + strandat 76.418 kb on - strand, within GFF356at 76.593 kb on - strand, within GFF356at 76.899 kb on - strandat 76.970 kb on - strandat 76.987 kb on - strandat 77.002 kb on - strandat 77.005 kb on - strandat 77.062 kb on - strandat 77.202 kb on + strand, within GFF357at 77.203 kb on - strand, within GFF357

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI
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75,430 + GFF355 0.63 -0.6
75,699 + GFF355 0.76 +0.1
76,128 + -1.7
76,418 - GFF356 0.31 -0.8
76,593 - GFF356 0.57 -0.4
76,899 - -0.0
76,970 - +0.3
76,987 - +0.9
77,002 - -0.2
77,005 - -0.5
77,062 - -0.4
77,202 + GFF357 0.14 +3.3
77,203 - GFF357 0.14 +0.1

Or see this region's nucleotide sequence