Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3147 and GFF3148 overlap by 4 nucleotides GFF3148 and GFF3149 are separated by 206 nucleotides GFF3149 and GFF3150 are separated by 212 nucleotides GFF3150 and GFF3151 are separated by 5 nucleotides
GFF3147 - FIG074102: hypothetical protein, at 152,450 to 152,872
GFF3147
GFF3148 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 152,869 to 153,501
GFF3148
GFF3149 - Type I secretion outer membrane protein, TolC precursor, at 153,708 to 155,177
GFF3149
GFF3150 - UPF0441 protein ygiB, at 155,390 to 156,061
GFF3150
GFF3151 - Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1, at 156,067 to 157,230
GFF3151
Position (kb) 153
154
155
156 Strain fitness (log2 ratio) -1
0
1
2 at 152.744 kb on + strand, within GFF3147 at 153.007 kb on - strand, within GFF3148 at 153.037 kb on - strand, within GFF3148 at 153.076 kb on - strand, within GFF3148 at 153.076 kb on - strand, within GFF3148 at 153.113 kb on + strand, within GFF3148 at 153.205 kb on + strand, within GFF3148 at 153.522 kb on - strand at 153.529 kb on - strand at 153.529 kb on - strand at 153.603 kb on - strand at 153.684 kb on - strand at 153.763 kb on + strand at 153.764 kb on - strand at 153.921 kb on + strand, within GFF3149 at 153.950 kb on + strand, within GFF3149 at 153.998 kb on - strand, within GFF3149 at 154.005 kb on - strand, within GFF3149 at 154.065 kb on + strand, within GFF3149 at 154.116 kb on - strand, within GFF3149 at 154.146 kb on + strand, within GFF3149 at 154.169 kb on + strand, within GFF3149 at 154.174 kb on - strand, within GFF3149 at 154.255 kb on - strand, within GFF3149 at 154.430 kb on + strand, within GFF3149 at 154.658 kb on + strand, within GFF3149 at 154.683 kb on + strand, within GFF3149 at 154.683 kb on + strand, within GFF3149 at 154.744 kb on + strand, within GFF3149 at 154.744 kb on + strand, within GFF3149 at 154.744 kb on + strand, within GFF3149 at 154.744 kb on + strand, within GFF3149 at 154.757 kb on + strand, within GFF3149 at 154.763 kb on + strand, within GFF3149 at 154.795 kb on - strand, within GFF3149 at 154.795 kb on - strand, within GFF3149 at 154.796 kb on + strand, within GFF3149 at 154.962 kb on - strand, within GFF3149 at 155.015 kb on + strand, within GFF3149 at 155.016 kb on - strand, within GFF3149 at 155.094 kb on + strand at 155.097 kb on - strand at 155.179 kb on - strand at 155.221 kb on + strand at 155.222 kb on - strand at 155.251 kb on + strand at 155.251 kb on + strand at 155.270 kb on - strand at 155.280 kb on - strand at 155.361 kb on + strand at 155.361 kb on + strand at 155.362 kb on - strand at 155.362 kb on - strand at 155.365 kb on + strand at 155.389 kb on + strand at 155.486 kb on - strand, within GFF3150 at 155.630 kb on - strand, within GFF3150 at 155.634 kb on - strand, within GFF3150 at 155.869 kb on + strand, within GFF3150 at 155.869 kb on + strand, within GFF3150 at 155.884 kb on + strand, within GFF3150 at 156.062 kb on + strand at 156.082 kb on - strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.146 kb on - strand at 156.175 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 152,744 + GFF3147 0.70 +1.4 153,007 - GFF3148 0.22 -0.2 153,037 - GFF3148 0.27 +0.1 153,076 - GFF3148 0.33 -0.4 153,076 - GFF3148 0.33 +0.1 153,113 + GFF3148 0.39 +0.8 153,205 + GFF3148 0.53 -0.7 153,522 - +0.1 153,529 - -0.3 153,529 - +0.7 153,603 - -0.2 153,684 - -0.2 153,763 + -0.1 153,764 - +1.4 153,921 + GFF3149 0.14 -1.0 153,950 + GFF3149 0.16 +0.5 153,998 - GFF3149 0.20 -1.7 154,005 - GFF3149 0.20 -0.1 154,065 + GFF3149 0.24 -0.6 154,116 - GFF3149 0.28 -0.6 154,146 + GFF3149 0.30 -1.4 154,169 + GFF3149 0.31 -0.8 154,174 - GFF3149 0.32 -0.1 154,255 - GFF3149 0.37 -0.5 154,430 + GFF3149 0.49 +0.7 154,658 + GFF3149 0.65 -0.5 154,683 + GFF3149 0.66 -0.6 154,683 + GFF3149 0.66 +0.6 154,744 + GFF3149 0.70 -0.5 154,744 + GFF3149 0.70 -0.8 154,744 + GFF3149 0.70 -0.3 154,744 + GFF3149 0.70 -0.2 154,757 + GFF3149 0.71 +0.7 154,763 + GFF3149 0.72 -1.2 154,795 - GFF3149 0.74 +0.3 154,795 - GFF3149 0.74 -1.0 154,796 + GFF3149 0.74 +0.4 154,962 - GFF3149 0.85 -0.7 155,015 + GFF3149 0.89 -0.4 155,016 - GFF3149 0.89 -0.6 155,094 + +0.3 155,097 - +0.3 155,179 - +0.3 155,221 + -0.0 155,222 - -0.2 155,251 + +0.3 155,251 + +0.3 155,270 - -0.5 155,280 - -0.7 155,361 + -1.1 155,361 + +0.0 155,362 - +0.1 155,362 - -0.2 155,365 + -0.1 155,389 + +0.9 155,486 - GFF3150 0.14 +0.2 155,630 - GFF3150 0.36 +1.1 155,634 - GFF3150 0.36 +0.2 155,869 + GFF3150 0.71 +0.6 155,869 + GFF3150 0.71 -0.0 155,884 + GFF3150 0.74 -0.3 156,062 + +0.3 156,082 - +0.4 156,141 + +2.2 156,141 + +0.4 156,141 + -0.0 156,141 + -0.0 156,141 + +1.0 156,141 + -0.4 156,146 - -0.2 156,175 + -0.6
Or see this region's nucleotide sequence