Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3101 and GFF3102 are separated by 27 nucleotides GFF3102 and GFF3103 overlap by 8 nucleotides GFF3103 and GFF3104 overlap by 8 nucleotides GFF3104 and GFF3105 overlap by 1 nucleotides
GFF3101 - [NiFe] hydrogenase metallocenter assembly protein HybG, at 112,337 to 112,585
GFF3101
GFF3102 - [NiFe] hydrogenase nickel incorporation protein HybF, at 112,613 to 112,954
GFF3102
GFF3103 - Hydrogenase-2 operon protein hybE, at 112,947 to 113,435
GFF3103
GFF3104 - Hydrogenase maturation protease (EC 3.4.24.-), at 113,428 to 113,922
GFF3104
GFF3105 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 113,922 to 115,625
GFF3105
Position (kb)
113
114 Strain fitness (log2 ratio)
-1
0
1 at 112.433 kb on + strand, within GFF3101 at 112.434 kb on - strand, within GFF3101 at 112.434 kb on - strand, within GFF3101 at 112.489 kb on + strand, within GFF3101 at 112.541 kb on - strand, within GFF3101 at 112.579 kb on + strand at 112.586 kb on - strand at 112.628 kb on - strand at 112.857 kb on - strand, within GFF3102 at 112.869 kb on - strand, within GFF3102 at 112.915 kb on + strand, within GFF3102 at 113.031 kb on + strand, within GFF3103 at 113.325 kb on - strand, within GFF3103 at 113.834 kb on + strand, within GFF3104 at 113.911 kb on + strand at 113.924 kb on - strand at 114.015 kb on + strand at 114.016 kb on - strand at 114.098 kb on + strand, within GFF3105 at 114.099 kb on - strand, within GFF3105 at 114.139 kb on + strand, within GFF3105 at 114.139 kb on + strand, within GFF3105 at 114.144 kb on - strand, within GFF3105 at 114.150 kb on - strand, within GFF3105 at 114.278 kb on + strand, within GFF3105 at 114.282 kb on + strand, within GFF3105 at 114.283 kb on - strand, within GFF3105 at 114.298 kb on + strand, within GFF3105 at 114.312 kb on + strand, within GFF3105 at 114.312 kb on + strand, within GFF3105 at 114.366 kb on - strand, within GFF3105 at 114.542 kb on - strand, within GFF3105 at 114.542 kb on - strand, within GFF3105 at 114.542 kb on - strand, within GFF3105 at 114.713 kb on + strand, within GFF3105 at 114.718 kb on + strand, within GFF3105 at 114.820 kb on + strand, within GFF3105 at 114.852 kb on - strand, within GFF3105 at 114.854 kb on - strand, within GFF3105 at 114.901 kb on + strand, within GFF3105
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 112,433 + GFF3101 0.39 -0.5 112,434 - GFF3101 0.39 +1.7 112,434 - GFF3101 0.39 +0.6 112,489 + GFF3101 0.61 -0.2 112,541 - GFF3101 0.82 +1.2 112,579 + +0.4 112,586 - +0.4 112,628 - +0.6 112,857 - GFF3102 0.71 +0.6 112,869 - GFF3102 0.75 +0.3 112,915 + GFF3102 0.88 -0.1 113,031 + GFF3103 0.17 -0.9 113,325 - GFF3103 0.77 +0.9 113,834 + GFF3104 0.82 -0.9 113,911 + -0.0 113,924 - +1.1 114,015 + +0.2 114,016 - -0.5 114,098 + GFF3105 0.10 +0.6 114,099 - GFF3105 0.10 +0.1 114,139 + GFF3105 0.13 -0.6 114,139 + GFF3105 0.13 -0.4 114,144 - GFF3105 0.13 +0.9 114,150 - GFF3105 0.13 -0.4 114,278 + GFF3105 0.21 +0.5 114,282 + GFF3105 0.21 +0.4 114,283 - GFF3105 0.21 +1.1 114,298 + GFF3105 0.22 -0.1 114,312 + GFF3105 0.23 +0.0 114,312 + GFF3105 0.23 +1.5 114,366 - GFF3105 0.26 +0.5 114,542 - GFF3105 0.36 +0.3 114,542 - GFF3105 0.36 -0.4 114,542 - GFF3105 0.36 +0.1 114,713 + GFF3105 0.46 -0.1 114,718 + GFF3105 0.47 +0.8 114,820 + GFF3105 0.53 +0.3 114,852 - GFF3105 0.55 +0.1 114,854 - GFF3105 0.55 -0.2 114,901 + GFF3105 0.57 -0.2
Or see this region's nucleotide sequence