Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2989 and GFF2990 are separated by 5 nucleotides GFF2990 and GFF2991 are separated by 23 nucleotides GFF2991 and GFF2992 are separated by 112 nucleotides GFF2992 and GFF2993 are separated by 52 nucleotides
GFF2989 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 7,083 to 8,816
GFF2989
GFF2990 - Thiol:disulfide interchange protein DsbC, at 8,822 to 9,535
GFF2990
GFF2991 - Tyrosine recombinase XerD, at 9,559 to 10,455
GFF2991
GFF2992 - Flavodoxin 2, at 10,568 to 11,089
GFF2992
GFF2993 - FIG00731769: possible membrane protein, at 11,142 to 11,555
GFF2993
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 8.730 kb on + strand at 8.742 kb on + strand at 8.742 kb on + strand at 8.742 kb on + strand at 8.824 kb on - strand at 8.824 kb on - strand at 8.824 kb on - strand at 8.901 kb on + strand, within GFF2990 at 8.902 kb on - strand, within GFF2990 at 8.916 kb on - strand, within GFF2990 at 8.961 kb on + strand, within GFF2990 at 9.007 kb on + strand, within GFF2990 at 9.058 kb on + strand, within GFF2990 at 9.068 kb on - strand, within GFF2990 at 9.114 kb on + strand, within GFF2990 at 9.139 kb on + strand, within GFF2990 at 9.161 kb on + strand, within GFF2990 at 9.161 kb on + strand, within GFF2990 at 9.162 kb on - strand, within GFF2990 at 9.162 kb on - strand, within GFF2990 at 9.231 kb on + strand, within GFF2990 at 9.337 kb on + strand, within GFF2990 at 9.337 kb on + strand, within GFF2990 at 9.338 kb on - strand, within GFF2990 at 9.361 kb on - strand, within GFF2990 at 9.718 kb on + strand, within GFF2991 at 9.719 kb on - strand, within GFF2991 at 9.719 kb on - strand, within GFF2991 at 9.767 kb on + strand, within GFF2991 at 9.856 kb on + strand, within GFF2991 at 10.028 kb on + strand, within GFF2991 at 10.044 kb on + strand, within GFF2991 at 10.045 kb on - strand, within GFF2991 at 10.491 kb on + strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.543 kb on + strand at 10.561 kb on + strand at 10.562 kb on - strand at 10.562 kb on - strand at 10.567 kb on - strand at 10.567 kb on - strand at 10.604 kb on + strand at 10.604 kb on + strand at 10.662 kb on + strand, within GFF2992 at 10.662 kb on + strand, within GFF2992 at 10.662 kb on + strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.663 kb on - strand, within GFF2992 at 10.703 kb on + strand, within GFF2992 at 10.873 kb on + strand, within GFF2992 at 10.907 kb on - strand, within GFF2992 at 10.954 kb on + strand, within GFF2992 at 11.035 kb on + strand, within GFF2992 at 11.036 kb on - strand, within GFF2992 at 11.105 kb on - strand at 11.170 kb on - strand at 11.287 kb on - strand, within GFF2993 at 11.375 kb on + strand, within GFF2993 at 11.375 kb on + strand, within GFF2993 at 11.376 kb on - strand, within GFF2993 at 11.390 kb on + strand, within GFF2993 at 11.422 kb on - strand, within GFF2993 at 11.422 kb on - strand, within GFF2993 at 11.422 kb on - strand, within GFF2993 at 11.424 kb on + strand, within GFF2993 at 11.424 kb on + strand, within GFF2993 at 11.425 kb on - strand, within GFF2993 at 11.444 kb on - strand, within GFF2993
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 8,730 + +0.4 8,742 + +0.2 8,742 + +0.1 8,742 + -0.6 8,824 - -0.0 8,824 - +0.1 8,824 - -0.1 8,901 + GFF2990 0.11 -0.0 8,902 - GFF2990 0.11 -1.1 8,916 - GFF2990 0.13 -0.1 8,961 + GFF2990 0.19 -2.2 9,007 + GFF2990 0.26 +1.1 9,058 + GFF2990 0.33 +0.3 9,068 - GFF2990 0.34 -0.2 9,114 + GFF2990 0.41 -0.2 9,139 + GFF2990 0.44 +0.3 9,161 + GFF2990 0.47 -1.0 9,161 + GFF2990 0.47 +0.3 9,162 - GFF2990 0.48 +0.3 9,162 - GFF2990 0.48 -0.4 9,231 + GFF2990 0.57 +0.1 9,337 + GFF2990 0.72 -0.6 9,337 + GFF2990 0.72 -0.1 9,338 - GFF2990 0.72 -0.3 9,361 - GFF2990 0.75 +0.3 9,718 + GFF2991 0.18 +0.7 9,719 - GFF2991 0.18 -0.1 9,719 - GFF2991 0.18 +0.3 9,767 + GFF2991 0.23 -0.9 9,856 + GFF2991 0.33 -0.5 10,028 + GFF2991 0.52 -0.5 10,044 + GFF2991 0.54 -1.9 10,045 - GFF2991 0.54 -3.1 10,491 + +0.9 10,492 - +0.1 10,492 - +0.7 10,492 - -0.3 10,492 - -0.1 10,543 + +0.2 10,561 + -2.2 10,562 - +0.0 10,562 - -0.5 10,567 - -0.6 10,567 - +0.7 10,604 + -1.1 10,604 + +0.2 10,662 + GFF2992 0.18 +0.0 10,662 + GFF2992 0.18 +1.7 10,662 + GFF2992 0.18 +0.4 10,663 - GFF2992 0.18 -0.1 10,663 - GFF2992 0.18 -0.3 10,663 - GFF2992 0.18 +0.0 10,663 - GFF2992 0.18 +0.0 10,703 + GFF2992 0.26 +0.9 10,873 + GFF2992 0.58 +0.3 10,907 - GFF2992 0.65 +0.1 10,954 + GFF2992 0.74 +2.7 11,035 + GFF2992 0.89 +0.9 11,036 - GFF2992 0.90 +1.4 11,105 - +2.9 11,170 - +0.1 11,287 - GFF2993 0.35 -0.7 11,375 + GFF2993 0.56 +2.2 11,375 + GFF2993 0.56 +0.2 11,376 - GFF2993 0.57 +0.2 11,390 + GFF2993 0.60 -1.1 11,422 - GFF2993 0.68 -0.4 11,422 - GFF2993 0.68 +1.5 11,422 - GFF2993 0.68 -0.3 11,424 + GFF2993 0.68 -0.3 11,424 + GFF2993 0.68 -0.1 11,425 - GFF2993 0.68 +0.2 11,444 - GFF2993 0.73 +0.5
Or see this region's nucleotide sequence