Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2952 and GFF2953 overlap by 4 nucleotides GFF2953 and GFF2954 are separated by 226 nucleotides GFF2954 and GFF2955 are separated by 93 nucleotides
GFF2952 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 30,363 to 31,091
GFF2952
GFF2953 - Sigma cross-reacting protein 27A, at 31,088 to 31,741
GFF2953
GFF2954 - Aerobic respiration control sensor protein arcB (EC 2.7.3.-), at 31,968 to 34,304
GFF2954
GFF2955 - COG1242: Predicted Fe-S oxidoreductase, at 34,398 to 35,327
GFF2955
Position (kb)
31
32
33
34
35 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 31.042 kb on - strand at 31.048 kb on - strand at 31.119 kb on - strand at 31.165 kb on + strand, within GFF2953 at 31.167 kb on + strand, within GFF2953 at 31.211 kb on - strand, within GFF2953 at 31.230 kb on - strand, within GFF2953 at 31.230 kb on - strand, within GFF2953 at 31.283 kb on + strand, within GFF2953 at 31.725 kb on - strand at 31.852 kb on + strand at 31.852 kb on + strand at 31.901 kb on + strand at 31.901 kb on + strand at 32.128 kb on - strand at 32.148 kb on - strand at 32.153 kb on + strand at 32.287 kb on + strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 32.945 kb on - strand, within GFF2954 at 33.031 kb on + strand, within GFF2954 at 33.066 kb on - strand, within GFF2954 at 33.279 kb on - strand, within GFF2954 at 33.341 kb on + strand, within GFF2954 at 33.440 kb on + strand, within GFF2954 at 33.441 kb on - strand, within GFF2954 at 33.447 kb on - strand, within GFF2954 at 33.447 kb on - strand, within GFF2954 at 33.498 kb on - strand, within GFF2954 at 33.560 kb on - strand, within GFF2954 at 33.649 kb on - strand, within GFF2954 at 33.695 kb on - strand, within GFF2954 at 33.695 kb on - strand, within GFF2954 at 33.695 kb on - strand, within GFF2954 at 33.856 kb on - strand, within GFF2954 at 33.959 kb on + strand, within GFF2954 at 33.992 kb on + strand, within GFF2954 at 33.993 kb on - strand, within GFF2954 at 34.093 kb on + strand at 34.114 kb on - strand at 34.114 kb on - strand at 34.274 kb on - strand at 34.274 kb on - strand at 34.276 kb on + strand at 34.276 kb on + strand at 34.277 kb on - strand at 34.305 kb on - strand at 34.334 kb on + strand at 34.345 kb on + strand at 34.363 kb on + strand at 34.376 kb on - strand at 34.405 kb on - strand at 34.463 kb on - strand at 34.556 kb on - strand, within GFF2955 at 34.561 kb on - strand, within GFF2955 at 34.676 kb on + strand, within GFF2955 at 34.676 kb on + strand, within GFF2955 at 34.677 kb on - strand, within GFF2955 at 34.695 kb on - strand, within GFF2955 at 34.947 kb on + strand, within GFF2955 at 34.950 kb on + strand, within GFF2955 at 35.026 kb on + strand, within GFF2955 at 35.027 kb on - strand, within GFF2955 at 35.080 kb on + strand, within GFF2955 at 35.234 kb on + strand, within GFF2955 at 35.258 kb on + strand at 35.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 31,042 - +0.8 31,048 - +0.6 31,119 - +0.3 31,165 + GFF2953 0.12 +0.2 31,167 + GFF2953 0.12 +0.3 31,211 - GFF2953 0.19 +1.7 31,230 - GFF2953 0.22 -0.1 31,230 - GFF2953 0.22 -0.1 31,283 + GFF2953 0.30 -0.2 31,725 - +0.1 31,852 + +0.6 31,852 + -0.6 31,901 + +0.3 31,901 + +0.0 32,128 - -1.3 32,148 - -0.8 32,153 + -2.0 32,287 + GFF2954 0.14 -1.6 32,945 - GFF2954 0.42 -1.6 32,945 - GFF2954 0.42 -1.6 32,945 - GFF2954 0.42 +0.6 32,945 - GFF2954 0.42 -3.7 32,945 - GFF2954 0.42 -1.3 32,945 - GFF2954 0.42 +1.1 32,945 - GFF2954 0.42 -0.6 33,031 + GFF2954 0.45 -1.3 33,066 - GFF2954 0.47 -1.4 33,279 - GFF2954 0.56 -0.4 33,341 + GFF2954 0.59 -0.8 33,440 + GFF2954 0.63 +0.1 33,441 - GFF2954 0.63 -0.0 33,447 - GFF2954 0.63 -1.4 33,447 - GFF2954 0.63 -0.3 33,498 - GFF2954 0.65 -0.3 33,560 - GFF2954 0.68 -1.0 33,649 - GFF2954 0.72 -0.8 33,695 - GFF2954 0.74 -0.6 33,695 - GFF2954 0.74 -0.7 33,695 - GFF2954 0.74 -1.6 33,856 - GFF2954 0.81 -0.2 33,959 + GFF2954 0.85 -1.2 33,992 + GFF2954 0.87 -0.7 33,993 - GFF2954 0.87 -1.6 34,093 + -0.9 34,114 - -1.0 34,114 - -1.0 34,274 - +0.1 34,274 - -0.4 34,276 + -0.9 34,276 + -0.5 34,277 - -0.3 34,305 - -0.8 34,334 + -0.8 34,345 + +0.3 34,363 + -0.4 34,376 - -0.1 34,405 - -1.1 34,463 - -0.1 34,556 - GFF2955 0.17 +0.4 34,561 - GFF2955 0.18 -0.7 34,676 + GFF2955 0.30 +0.6 34,676 + GFF2955 0.30 -0.2 34,677 - GFF2955 0.30 +1.3 34,695 - GFF2955 0.32 -1.6 34,947 + GFF2955 0.59 +0.1 34,950 + GFF2955 0.59 +0.7 35,026 + GFF2955 0.68 +0.3 35,027 - GFF2955 0.68 +1.1 35,080 + GFF2955 0.73 -0.0 35,234 + GFF2955 0.90 +0.3 35,258 + -0.5 35,304 - +0.4
Or see this region's nucleotide sequence