Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2638 and GFF2639 are separated by 110 nucleotides GFF2639 and GFF2640 are separated by 43 nucleotides GFF2640 and GFF2641 are separated by 38 nucleotides GFF2641 and GFF2642 are separated by 105 nucleotides
GFF2638 - D-lactate dehydrogenase (EC 1.1.1.28), at 165,647 to 166,636
GFF2638
GFF2639 - Heat shock protein hslJ, at 166,747 to 167,157
GFF2639
GFF2640 - probable lipoprotein YPO2331, at 167,201 to 167,467
GFF2640
GFF2641 - hypothetical protein, at 167,506 to 167,637
GFF2641
GFF2642 - Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-), at 167,743 to 171,267
GFF2642
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1 at 166.337 kb on - strand, within GFF2638 at 166.339 kb on + strand, within GFF2638 at 166.339 kb on + strand, within GFF2638 at 166.340 kb on - strand, within GFF2638 at 166.634 kb on + strand at 166.706 kb on + strand at 166.710 kb on - strand at 166.721 kb on + strand at 166.742 kb on + strand at 167.029 kb on - strand, within GFF2639 at 167.131 kb on + strand at 167.131 kb on + strand at 167.177 kb on + strand at 167.429 kb on + strand, within GFF2640 at 167.430 kb on - strand, within GFF2640 at 167.508 kb on - strand at 167.512 kb on - strand at 167.575 kb on + strand, within GFF2641 at 167.731 kb on + strand at 167.731 kb on + strand at 167.742 kb on + strand at 167.875 kb on - strand at 168.023 kb on - strand at 168.023 kb on - strand at 168.024 kb on + strand at 168.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 166,337 - GFF2638 0.70 -0.8 166,339 + GFF2638 0.70 +1.5 166,339 + GFF2638 0.70 -0.6 166,340 - GFF2638 0.70 +0.5 166,634 + +0.5 166,706 + -1.3 166,710 - +0.4 166,721 + -0.5 166,742 + -0.1 167,029 - GFF2639 0.69 -0.5 167,131 + -0.3 167,131 + +0.5 167,177 + -0.2 167,429 + GFF2640 0.85 -0.8 167,430 - GFF2640 0.86 +1.0 167,508 - +0.2 167,512 - -0.0 167,575 + GFF2641 0.52 +0.1 167,731 + -0.4 167,731 + +0.4 167,742 + +0.1 167,875 - +0.4 168,023 - -0.2 168,023 - +0.5 168,024 + +1.1 168,054 - -0.0
Or see this region's nucleotide sequence