Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2620

Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2618 and GFF2619 are separated by 762 nucleotidesGFF2619 and GFF2620 are separated by 19 nucleotidesGFF2620 and GFF2621 overlap by 11 nucleotides GFF2618 - FrmR: Negative transcriptional regulator of formaldehyde detoxification operon, at 144,441 to 144,716 GFF2618 GFF2619 - hypothetical protein, at 145,479 to 145,604 GFF2619 GFF2620 - FIG01045674: hypothetical protein, at 145,624 to 145,779 GFF2620 GFF2621 - FIG01045328: hypothetical protein, at 145,769 to 146,485 GFF2621 Position (kb) 145 146Strain fitness (log2 ratio) -1 0 1at 144.686 kb on + strand, within GFF2618at 144.733 kb on - strandat 144.946 kb on + strandat 145.475 kb on + strandat 145.722 kb on - strand, within GFF2620at 146.379 kb on + strand, within GFF2621

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI
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144,686 + GFF2618 0.89 +1.1
144,733 - +0.5
144,946 + -0.9
145,475 + +0.1
145,722 - GFF2620 0.63 -0.4
146,379 + GFF2621 0.85 +0.2

Or see this region's nucleotide sequence