Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF234 and GFF235 are separated by 258 nucleotides GFF235 and GFF236 are separated by 185 nucleotides GFF236 and GFF237 are separated by 36 nucleotides GFF237 and GFF238 overlap by 4 nucleotides
GFF234 - STMD1.44 protein, at 9,771 to 10,160
GFF234
GFF235 - possible exported protein, at 10,419 to 10,670
GFF235
GFF236 - FIG00545237: hypothetical protein, at 10,856 to 11,059
GFF236
GFF237 - Diaminopimelate epimerase (EC 5.1.1.7), at 11,096 to 11,920
GFF237
GFF238 - Protein of unknown function DUF484, at 11,917 to 12,624
GFF238
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1 at 10.002 kb on + strand, within GFF234 at 10.049 kb on + strand, within GFF234 at 10.062 kb on - strand, within GFF234 at 10.069 kb on + strand, within GFF234 at 10.070 kb on - strand, within GFF234 at 10.118 kb on + strand, within GFF234 at 10.192 kb on + strand at 10.204 kb on - strand at 10.225 kb on + strand at 10.290 kb on - strand at 10.549 kb on + strand, within GFF235 at 10.670 kb on + strand at 10.670 kb on + strand at 10.689 kb on + strand at 10.689 kb on + strand at 10.690 kb on - strand at 10.690 kb on - strand at 10.693 kb on + strand at 10.742 kb on - strand at 10.774 kb on + strand at 10.841 kb on + strand at 10.841 kb on + strand at 10.870 kb on + strand at 10.935 kb on - strand, within GFF236 at 11.054 kb on + strand at 11.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 10,002 + GFF234 0.59 +0.9 10,049 + GFF234 0.71 -1.3 10,062 - GFF234 0.75 +0.3 10,069 + GFF234 0.76 -0.5 10,070 - GFF234 0.77 +0.9 10,118 + GFF234 0.89 +0.2 10,192 + +0.5 10,204 - -0.4 10,225 + -0.2 10,290 - +0.2 10,549 + GFF235 0.52 -0.3 10,670 + -0.1 10,670 + +0.6 10,689 + +0.2 10,689 + +0.3 10,690 - +0.2 10,690 - +0.2 10,693 + +0.5 10,742 - +0.2 10,774 + -0.1 10,841 + +0.1 10,841 + +0.3 10,870 + -0.5 10,935 - GFF236 0.39 -1.2 11,054 + +0.0 11,917 - -2.1
Or see this region's nucleotide sequence