Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF229 and GFF230 are separated by 376 nucleotides GFF230 and GFF231 are separated by 107 nucleotides GFF231 and GFF232 are separated by 368 nucleotides
GFF229 - Porphobilinogen deaminase (EC 2.5.1.61), at 3,883 to 4,824
GFF229
GFF230 - Adenylate cyclase (EC 4.6.1.1), at 5,201 to 7,747
GFF230
GFF231 - Putative inner membrane protein, at 7,855 to 8,208
GFF231
GFF232 - Putative inner membrane protein, at 8,577 to 8,936
GFF232
Position (kb) 5
6
7
8 Strain fitness (log2 ratio) -2
-1
0
1 at 4.860 kb on - strand at 4.869 kb on - strand at 4.878 kb on - strand at 4.878 kb on - strand at 4.941 kb on - strand at 4.995 kb on + strand at 4.996 kb on - strand at 4.996 kb on - strand at 5.003 kb on + strand at 5.004 kb on - strand at 5.004 kb on - strand at 5.004 kb on - strand at 5.007 kb on + strand at 5.022 kb on + strand at 5.091 kb on + strand at 5.127 kb on + strand at 5.204 kb on + strand at 5.204 kb on + strand at 5.213 kb on - strand at 5.303 kb on + strand at 5.322 kb on + strand at 5.330 kb on + strand at 5.381 kb on - strand at 5.391 kb on - strand at 5.495 kb on + strand, within GFF230 at 5.605 kb on + strand, within GFF230 at 5.605 kb on + strand, within GFF230 at 5.605 kb on + strand, within GFF230 at 5.606 kb on - strand, within GFF230 at 5.606 kb on - strand, within GFF230 at 5.737 kb on + strand, within GFF230 at 5.738 kb on - strand, within GFF230 at 5.791 kb on + strand, within GFF230 at 5.803 kb on + strand, within GFF230 at 5.804 kb on - strand, within GFF230 at 5.841 kb on - strand, within GFF230 at 5.841 kb on - strand, within GFF230 at 5.869 kb on - strand, within GFF230 at 5.954 kb on + strand, within GFF230 at 5.955 kb on - strand, within GFF230 at 5.955 kb on - strand, within GFF230 at 6.023 kb on + strand, within GFF230 at 6.231 kb on + strand, within GFF230 at 6.252 kb on + strand, within GFF230 at 6.262 kb on - strand, within GFF230 at 6.386 kb on - strand, within GFF230 at 6.600 kb on - strand, within GFF230 at 6.664 kb on + strand, within GFF230 at 6.665 kb on - strand, within GFF230 at 6.667 kb on + strand, within GFF230 at 6.667 kb on + strand, within GFF230 at 6.727 kb on + strand, within GFF230 at 6.727 kb on + strand, within GFF230 at 6.728 kb on - strand, within GFF230 at 6.759 kb on + strand, within GFF230 at 6.830 kb on + strand, within GFF230 at 6.879 kb on - strand, within GFF230 at 6.905 kb on - strand, within GFF230 at 6.972 kb on + strand, within GFF230 at 6.991 kb on - strand, within GFF230 at 6.993 kb on - strand, within GFF230 at 7.191 kb on + strand, within GFF230 at 7.191 kb on + strand, within GFF230 at 7.192 kb on - strand, within GFF230 at 7.473 kb on - strand, within GFF230 at 7.503 kb on + strand at 7.522 kb on + strand at 7.523 kb on - strand at 7.587 kb on - strand at 7.610 kb on - strand at 7.796 kb on - strand at 7.918 kb on - strand, within GFF231 at 7.928 kb on + strand, within GFF231 at 7.928 kb on + strand, within GFF231 at 7.928 kb on + strand, within GFF231 at 8.098 kb on + strand, within GFF231 at 8.540 kb on + strand at 8.653 kb on + strand, within GFF232 at 8.653 kb on + strand, within GFF232 at 8.660 kb on - strand, within GFF232 at 8.726 kb on + strand, within GFF232
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 4,860 - +0.4 4,869 - +0.3 4,878 - -0.6 4,878 - +0.1 4,941 - +0.2 4,995 + -0.2 4,996 - +0.3 4,996 - -0.1 5,003 + -0.4 5,004 - +1.0 5,004 - +0.5 5,004 - -0.1 5,007 + +0.6 5,022 + +0.1 5,091 + +1.1 5,127 + +1.0 5,204 + -0.6 5,204 + -1.8 5,213 - -1.0 5,303 + +0.2 5,322 + -1.0 5,330 + -1.4 5,381 - +1.2 5,391 - -0.6 5,495 + GFF230 0.12 -0.4 5,605 + GFF230 0.16 -1.5 5,605 + GFF230 0.16 -0.8 5,605 + GFF230 0.16 -1.2 5,606 - GFF230 0.16 +0.7 5,606 - GFF230 0.16 -1.8 5,737 + GFF230 0.21 -1.2 5,738 - GFF230 0.21 +0.6 5,791 + GFF230 0.23 +0.0 5,803 + GFF230 0.24 -1.2 5,804 - GFF230 0.24 -1.4 5,841 - GFF230 0.25 -0.6 5,841 - GFF230 0.25 +0.2 5,869 - GFF230 0.26 -1.4 5,954 + GFF230 0.30 -0.9 5,955 - GFF230 0.30 -2.3 5,955 - GFF230 0.30 +0.8 6,023 + GFF230 0.32 +1.0 6,231 + GFF230 0.40 -1.3 6,252 + GFF230 0.41 -1.4 6,262 - GFF230 0.42 -1.6 6,386 - GFF230 0.47 -2.1 6,600 - GFF230 0.55 +1.2 6,664 + GFF230 0.57 -0.7 6,665 - GFF230 0.57 -2.0 6,667 + GFF230 0.58 -0.8 6,667 + GFF230 0.58 +0.4 6,727 + GFF230 0.60 -0.8 6,727 + GFF230 0.60 -0.6 6,728 - GFF230 0.60 -1.8 6,759 + GFF230 0.61 -0.2 6,830 + GFF230 0.64 +0.4 6,879 - GFF230 0.66 +0.2 6,905 - GFF230 0.67 -1.1 6,972 + GFF230 0.70 +1.2 6,991 - GFF230 0.70 -0.5 6,993 - GFF230 0.70 -1.4 7,191 + GFF230 0.78 +0.0 7,191 + GFF230 0.78 -0.6 7,192 - GFF230 0.78 -2.5 7,473 - GFF230 0.89 +0.6 7,503 + -0.1 7,522 + -1.0 7,523 - -0.1 7,587 - +0.5 7,610 - -1.5 7,796 - -0.1 7,918 - GFF231 0.18 +0.5 7,928 + GFF231 0.21 -0.2 7,928 + GFF231 0.21 +0.2 7,928 + GFF231 0.21 +0.2 8,098 + GFF231 0.69 +0.2 8,540 + +0.7 8,653 + GFF232 0.21 +0.9 8,653 + GFF232 0.21 +0.3 8,660 - GFF232 0.23 -1.1 8,726 + GFF232 0.41 -0.3
Or see this region's nucleotide sequence