Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2283 and GFF2284 are separated by 203 nucleotides GFF2284 and GFF2285 are separated by 100 nucleotides
GFF2283 - 6-phosphofructokinase (EC 2.7.1.11), at 71,254 to 72,216
GFF2283
GFF2284 - Sulfate-binding protein Sbp, at 72,420 to 73,409
GFF2284
GFF2285 - CDP-diacylglycerol pyrophosphatase (EC 3.6.1.26), at 73,510 to 74,265
GFF2285
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2 at 71.426 kb on + strand, within GFF2283 at 71.426 kb on + strand, within GFF2283 at 71.572 kb on + strand, within GFF2283 at 71.572 kb on + strand, within GFF2283 at 71.573 kb on - strand, within GFF2283 at 71.574 kb on + strand, within GFF2283 at 71.574 kb on + strand, within GFF2283 at 71.575 kb on - strand, within GFF2283 at 71.665 kb on - strand, within GFF2283 at 71.743 kb on - strand, within GFF2283 at 71.748 kb on + strand, within GFF2283 at 71.820 kb on + strand, within GFF2283 at 71.925 kb on + strand, within GFF2283 at 71.925 kb on + strand, within GFF2283 at 71.955 kb on + strand, within GFF2283 at 72.032 kb on - strand, within GFF2283 at 72.032 kb on - strand, within GFF2283 at 72.070 kb on - strand, within GFF2283 at 72.405 kb on + strand at 72.405 kb on + strand at 72.406 kb on - strand at 72.449 kb on - strand at 72.449 kb on - strand at 72.504 kb on + strand at 72.635 kb on + strand, within GFF2284 at 72.753 kb on + strand, within GFF2284 at 72.754 kb on - strand, within GFF2284 at 73.010 kb on + strand, within GFF2284 at 73.010 kb on + strand, within GFF2284 at 73.089 kb on - strand, within GFF2284 at 73.095 kb on - strand, within GFF2284 at 73.095 kb on - strand, within GFF2284 at 73.201 kb on - strand, within GFF2284 at 73.418 kb on + strand at 73.433 kb on - strand at 73.506 kb on + strand at 73.643 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.747 kb on + strand, within GFF2285 at 73.752 kb on + strand, within GFF2285 at 73.832 kb on - strand, within GFF2285 at 73.833 kb on + strand, within GFF2285 at 73.834 kb on - strand, within GFF2285 at 73.834 kb on - strand, within GFF2285 at 73.858 kb on - strand, within GFF2285 at 74.083 kb on - strand, within GFF2285 at 74.147 kb on + strand, within GFF2285 at 74.197 kb on - strand at 74.238 kb on + strand at 74.238 kb on + strand at 74.238 kb on + strand at 74.239 kb on - strand at 74.295 kb on - strand at 74.295 kb on - strand at 74.402 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 71,426 + GFF2283 0.18 +0.3 71,426 + GFF2283 0.18 -0.5 71,572 + GFF2283 0.33 -0.2 71,572 + GFF2283 0.33 -0.5 71,573 - GFF2283 0.33 -0.3 71,574 + GFF2283 0.33 +0.4 71,574 + GFF2283 0.33 -0.0 71,575 - GFF2283 0.33 -0.5 71,665 - GFF2283 0.43 -0.3 71,743 - GFF2283 0.51 +1.4 71,748 + GFF2283 0.51 -0.1 71,820 + GFF2283 0.59 +0.4 71,925 + GFF2283 0.70 -2.2 71,925 + GFF2283 0.70 -0.1 71,955 + GFF2283 0.73 -0.2 72,032 - GFF2283 0.81 -0.6 72,032 - GFF2283 0.81 +1.4 72,070 - GFF2283 0.85 -0.2 72,405 + +0.3 72,405 + +0.5 72,406 - -0.3 72,449 - +0.2 72,449 - +0.2 72,504 + +0.5 72,635 + GFF2284 0.22 +0.1 72,753 + GFF2284 0.34 +0.1 72,754 - GFF2284 0.34 -0.3 73,010 + GFF2284 0.60 -1.0 73,010 + GFF2284 0.60 -0.6 73,089 - GFF2284 0.68 -0.2 73,095 - GFF2284 0.68 +0.2 73,095 - GFF2284 0.68 -0.1 73,201 - GFF2284 0.79 -0.7 73,418 + -0.2 73,433 - +0.1 73,506 + +0.2 73,643 + GFF2285 0.18 +0.4 73,733 + GFF2285 0.29 +0.0 73,733 + GFF2285 0.29 -0.2 73,733 + GFF2285 0.29 -0.2 73,747 + GFF2285 0.31 -0.1 73,752 + GFF2285 0.32 +1.1 73,832 - GFF2285 0.43 +1.1 73,833 + GFF2285 0.43 -0.7 73,834 - GFF2285 0.43 +0.6 73,834 - GFF2285 0.43 -0.3 73,858 - GFF2285 0.46 +0.4 74,083 - GFF2285 0.76 +1.5 74,147 + GFF2285 0.84 +0.3 74,197 - +1.9 74,238 + -0.1 74,238 + +1.1 74,238 + +0.5 74,239 - -0.9 74,295 - +0.7 74,295 - -0.4 74,402 + -0.2
Or see this region's nucleotide sequence