Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2061 and GFF2062 are separated by 94 nucleotides GFF2062 and GFF2063 are separated by 61 nucleotides GFF2063 and GFF2064 overlap by 14 nucleotides GFF2064 and GFF2065 are separated by 212 nucleotides
GFF2061 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 25,647 to 26,546
GFF2061
GFF2062 - Predicted outer membrane lipoprotein YfeY, at 26,641 to 27,216
GFF2062
GFF2063 - Inner membrane protein YfeZ, at 27,278 to 27,727
GFF2063
GFF2064 - Acetyltransferase YpeA, at 27,714 to 28,139
GFF2064
GFF2065 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 28,352 to 29,221
GFF2065
Position (kb) 27
28 Strain fitness (log2 ratio) -1
0
1 at 26.303 kb on - strand, within GFF2061 at 26.746 kb on - strand, within GFF2062 at 27.025 kb on + strand, within GFF2062 at 27.241 kb on - strand at 27.583 kb on + strand, within GFF2063 at 27.787 kb on + strand, within GFF2064 at 27.787 kb on + strand, within GFF2064 at 27.787 kb on + strand, within GFF2064 at 27.788 kb on - strand, within GFF2064 at 27.788 kb on - strand, within GFF2064 at 27.810 kb on - strand, within GFF2064 at 27.928 kb on + strand, within GFF2064 at 27.928 kb on + strand, within GFF2064 at 28.131 kb on + strand at 28.177 kb on - strand at 28.267 kb on + strand at 28.317 kb on - strand at 28.436 kb on - strand at 28.436 kb on - strand at 28.450 kb on - strand, within GFF2065 at 28.718 kb on - strand, within GFF2065
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 26,303 - GFF2061 0.73 -0.3 26,746 - GFF2062 0.18 +0.6 27,025 + GFF2062 0.67 -0.1 27,241 - -0.3 27,583 + GFF2063 0.68 -1.0 27,787 + GFF2064 0.17 -1.5 27,787 + GFF2064 0.17 +0.0 27,787 + GFF2064 0.17 -0.4 27,788 - GFF2064 0.17 -0.1 27,788 - GFF2064 0.17 -0.1 27,810 - GFF2064 0.23 -0.8 27,928 + GFF2064 0.50 +0.1 27,928 + GFF2064 0.50 -1.4 28,131 + +0.1 28,177 - +0.2 28,267 + +0.5 28,317 - +0.1 28,436 - +0.0 28,436 - +0.5 28,450 - GFF2065 0.11 -0.1 28,718 - GFF2065 0.42 -0.5
Or see this region's nucleotide sequence