Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2031

Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2028 and GFF2029 are separated by 11 nucleotidesGFF2029 and GFF2030 are separated by 76 nucleotidesGFF2030 and GFF2031 are separated by 88 nucleotides GFF2028 - Type III secretion and flagellar regulator RtsA, at 34,717 to 35,592 GFF2028 GFF2029 - Flagellar regulon repressor RtsB, at 35,604 to 35,876 GFF2029 GFF2030 - FIG074102: hypothetical protein, at 35,953 to 36,114 GFF2030 GFF2031 - FIG01046611: hypothetical protein, at 36,203 to 36,391 GFF2031 Position (kb) 36 37Strain fitness (log2 ratio) -1 0 1at 35.990 kb on + strand, within GFF2030at 36.009 kb on - strand, within GFF2030at 36.024 kb on + strand, within GFF2030at 36.055 kb on - strand, within GFF2030at 36.069 kb on + strand, within GFF2030at 36.112 kb on + strandat 36.113 kb on - strandat 36.127 kb on + strandat 36.255 kb on - strand, within GFF2031at 36.392 kb on + strandat 36.396 kb on + strandat 36.397 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI
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35,990 + GFF2030 0.23 +0.0
36,009 - GFF2030 0.35 +0.7
36,024 + GFF2030 0.44 +0.1
36,055 - GFF2030 0.63 +0.3
36,069 + GFF2030 0.72 +0.7
36,112 + +0.1
36,113 - +0.7
36,127 + -1.0
36,255 - GFF2031 0.28 -0.5
36,392 + +0.4
36,396 + +0.7
36,397 - -0.4

Or see this region's nucleotide sequence