Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2026 and GFF2027 are separated by 394 nucleotides GFF2027 and GFF2028 are separated by 264 nucleotides GFF2028 and GFF2029 are separated by 11 nucleotides GFF2029 and GFF2030 are separated by 76 nucleotides GFF2030 and GFF2031 are separated by 88 nucleotides
GFF2026 - FIG032766: hypothetical protein, at 33,621 to 33,914
GFF2026
GFF2027 - putative cytoplasmic protein, at 34,309 to 34,452
GFF2027
GFF2028 - Type III secretion and flagellar regulator RtsA, at 34,717 to 35,592
GFF2028
GFF2029 - Flagellar regulon repressor RtsB, at 35,604 to 35,876
GFF2029
GFF2030 - FIG074102: hypothetical protein, at 35,953 to 36,114
GFF2030
GFF2031 - FIG01046611: hypothetical protein, at 36,203 to 36,391
GFF2031
Position (kb)
34
35
36 Strain fitness (log2 ratio)
-1
0
1 at 33.914 kb on + strand at 33.931 kb on - strand at 33.931 kb on - strand at 33.946 kb on - strand at 33.951 kb on + strand at 33.951 kb on + strand at 34.115 kb on + strand at 34.141 kb on + strand at 34.165 kb on - strand at 34.293 kb on - strand at 35.132 kb on + strand, within GFF2028 at 35.133 kb on - strand, within GFF2028 at 35.990 kb on + strand, within GFF2030 at 36.009 kb on - strand, within GFF2030 at 36.024 kb on + strand, within GFF2030 at 36.055 kb on - strand, within GFF2030 at 36.069 kb on + strand, within GFF2030 at 36.112 kb on + strand at 36.113 kb on - strand at 36.127 kb on + strand at 36.255 kb on - strand, within GFF2031 at 36.392 kb on + strand at 36.396 kb on + strand at 36.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 33,914 + +0.5 33,931 - +0.4 33,931 - +0.1 33,946 - -0.3 33,951 + +0.0 33,951 + +0.3 34,115 + +0.3 34,141 + -0.6 34,165 - +0.4 34,293 - +0.4 35,132 + GFF2028 0.47 -0.4 35,133 - GFF2028 0.47 -1.1 35,990 + GFF2030 0.23 +0.0 36,009 - GFF2030 0.35 +0.7 36,024 + GFF2030 0.44 +0.1 36,055 - GFF2030 0.63 +0.3 36,069 + GFF2030 0.72 +0.7 36,112 + +0.1 36,113 - +0.7 36,127 + -1.0 36,255 - GFF2031 0.28 -0.5 36,392 + +0.4 36,396 + +0.7 36,397 - -0.4
Or see this region's nucleotide sequence