Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1862 and GFF1863 overlap by 4 nucleotides GFF1863 and GFF1864 overlap by 1 nucleotides GFF1864 and GFF1865 overlap by 6 nucleotides GFF1865 and GFF1866 are separated by 154 nucleotides
GFF1862 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 55,361 to 57,007
GFF1862
GFF1863 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 57,004 to 57,339
GFF1863
GFF1864 - Polymyxin resistance protein PmrM, at 57,339 to 57,716
GFF1864
GFF1865 - Polymyxin resistance protein PmrD, at 57,711 to 57,911
GFF1865
GFF1866 - O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26), at 58,066 to 59,433
GFF1866
Position (kb) 57
58 Strain fitness (log2 ratio) -1
0
1 at 56.141 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.300 kb on + strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.301 kb on - strand, within GFF1862 at 56.362 kb on - strand, within GFF1862 at 56.382 kb on + strand, within GFF1862 at 56.577 kb on + strand, within GFF1862 at 56.578 kb on - strand, within GFF1862 at 56.581 kb on - strand, within GFF1862 at 56.707 kb on + strand, within GFF1862 at 56.708 kb on - strand, within GFF1862 at 56.771 kb on - strand, within GFF1862 at 56.771 kb on - strand, within GFF1862 at 56.809 kb on + strand, within GFF1862 at 56.809 kb on + strand, within GFF1862 at 56.809 kb on + strand, within GFF1862 at 56.810 kb on - strand, within GFF1862 at 56.810 kb on - strand, within GFF1862 at 56.890 kb on + strand at 56.960 kb on - strand at 56.967 kb on - strand at 56.985 kb on + strand at 57.137 kb on + strand, within GFF1863 at 57.487 kb on + strand, within GFF1864 at 57.755 kb on - strand, within GFF1865 at 57.856 kb on - strand, within GFF1865 at 57.856 kb on - strand, within GFF1865 at 58.119 kb on + strand at 58.235 kb on + strand, within GFF1866 at 58.236 kb on - strand, within GFF1866
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 56,141 + GFF1862 0.47 -0.4 56,300 + GFF1862 0.57 +0.6 56,300 + GFF1862 0.57 +0.3 56,300 + GFF1862 0.57 +0.3 56,300 + GFF1862 0.57 -0.1 56,301 - GFF1862 0.57 +1.4 56,301 - GFF1862 0.57 -1.0 56,301 - GFF1862 0.57 +0.4 56,301 - GFF1862 0.57 -0.0 56,301 - GFF1862 0.57 +0.6 56,362 - GFF1862 0.61 +0.5 56,382 + GFF1862 0.62 -1.4 56,577 + GFF1862 0.74 -0.2 56,578 - GFF1862 0.74 -0.2 56,581 - GFF1862 0.74 +0.2 56,707 + GFF1862 0.82 +0.3 56,708 - GFF1862 0.82 +0.5 56,771 - GFF1862 0.86 -0.2 56,771 - GFF1862 0.86 +0.9 56,809 + GFF1862 0.88 +0.3 56,809 + GFF1862 0.88 +1.0 56,809 + GFF1862 0.88 -0.1 56,810 - GFF1862 0.88 -0.2 56,810 - GFF1862 0.88 -0.3 56,890 + -1.3 56,960 - +0.3 56,967 - +0.9 56,985 + -0.1 57,137 + GFF1863 0.40 -0.4 57,487 + GFF1864 0.39 +0.3 57,755 - GFF1865 0.22 -0.3 57,856 - GFF1865 0.72 -0.1 57,856 - GFF1865 0.72 +0.2 58,119 + -0.4 58,235 + GFF1866 0.12 +0.8 58,236 - GFF1866 0.12 +0.1
Or see this region's nucleotide sequence