Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1856 and GFF1857 are separated by 44 nucleotides GFF1857 and GFF1858 are separated by 280 nucleotides GFF1858 and GFF1859 are separated by 2 nucleotides GFF1859 and GFF1860 overlap by 4 nucleotides
GFF1856 - Pyrimidine deoxynucleoside triphosphate (dYTP) pyrophosphohydrolase YfoO, at 48,990 to 49,415
GFF1856
GFF1857 - Polymyxin resistance protein PmrG; Ais protein, at 49,460 to 50,065
GFF1857
GFF1858 - UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC 2.6.1.-), at 50,346 to 51,503
GFF1858
GFF1859 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 51,506 to 52,489
GFF1859
GFF1860 - UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305) / UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13), at 52,486 to 54,468
GFF1860
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-1
0
1
2 at 49.362 kb on + strand, within GFF1856 at 49.411 kb on + strand at 49.412 kb on - strand at 49.742 kb on - strand, within GFF1857 at 50.101 kb on + strand at 50.102 kb on - strand at 50.108 kb on - strand at 50.114 kb on - strand at 50.175 kb on + strand at 50.594 kb on + strand, within GFF1858 at 50.619 kb on - strand, within GFF1858 at 50.623 kb on + strand, within GFF1858 at 50.702 kb on + strand, within GFF1858 at 50.738 kb on + strand, within GFF1858 at 50.751 kb on + strand, within GFF1858 at 50.784 kb on + strand, within GFF1858 at 50.784 kb on + strand, within GFF1858 at 50.851 kb on - strand, within GFF1858 at 51.276 kb on - strand, within GFF1858 at 51.276 kb on - strand, within GFF1858 at 51.347 kb on + strand, within GFF1858 at 51.360 kb on + strand, within GFF1858 at 51.491 kb on - strand at 51.502 kb on - strand at 51.502 kb on - strand at 51.555 kb on - strand at 51.643 kb on - strand, within GFF1859 at 51.938 kb on - strand, within GFF1859 at 52.024 kb on + strand, within GFF1859 at 52.025 kb on - strand, within GFF1859 at 52.140 kb on - strand, within GFF1859 at 52.148 kb on - strand, within GFF1859 at 52.169 kb on + strand, within GFF1859 at 52.169 kb on + strand, within GFF1859 at 52.170 kb on - strand, within GFF1859 at 52.267 kb on + strand, within GFF1859 at 52.268 kb on - strand, within GFF1859 at 52.315 kb on - strand, within GFF1859 at 52.315 kb on - strand, within GFF1859 at 52.319 kb on - strand, within GFF1859 at 52.385 kb on + strand, within GFF1859 at 52.386 kb on - strand, within GFF1859 at 52.400 kb on + strand at 52.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 49,362 + GFF1856 0.87 -0.9 49,411 + +0.4 49,412 - +0.4 49,742 - GFF1857 0.47 -0.4 50,101 + +0.3 50,102 - +0.9 50,108 - +0.6 50,114 - +0.2 50,175 + +0.5 50,594 + GFF1858 0.21 -0.0 50,619 - GFF1858 0.24 +2.0 50,623 + GFF1858 0.24 -0.8 50,702 + GFF1858 0.31 +0.8 50,738 + GFF1858 0.34 -1.0 50,751 + GFF1858 0.35 +1.0 50,784 + GFF1858 0.38 +1.2 50,784 + GFF1858 0.38 +0.4 50,851 - GFF1858 0.44 +0.0 51,276 - GFF1858 0.80 +0.1 51,276 - GFF1858 0.80 +1.1 51,347 + GFF1858 0.86 +0.8 51,360 + GFF1858 0.88 +0.0 51,491 - -1.2 51,502 - +1.1 51,502 - +1.0 51,555 - -0.3 51,643 - GFF1859 0.14 +0.6 51,938 - GFF1859 0.44 +0.8 52,024 + GFF1859 0.53 -0.3 52,025 - GFF1859 0.53 +0.7 52,140 - GFF1859 0.64 +0.4 52,148 - GFF1859 0.65 +0.2 52,169 + GFF1859 0.67 +1.8 52,169 + GFF1859 0.67 -0.4 52,170 - GFF1859 0.67 -0.5 52,267 + GFF1859 0.77 +0.0 52,268 - GFF1859 0.77 +0.2 52,315 - GFF1859 0.82 -0.5 52,315 - GFF1859 0.82 +2.3 52,319 - GFF1859 0.83 +0.1 52,385 + GFF1859 0.89 +0.6 52,386 - GFF1859 0.89 +0.3 52,400 + +0.1 52,401 - +0.2
Or see this region's nucleotide sequence