Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1628 and GFF1629 are separated by 99 nucleotides GFF1629 and GFF1630 are separated by 37 nucleotides
GFF1628 - Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-), at 12,085 to 13,263
GFF1628
GFF1629 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 13,363 to 14,004
GFF1629
GFF1630 - Phosphogluconate dehydratase (EC 4.2.1.12), at 14,042 to 15,853
GFF1630
Position (kb) 13
14
15 Strain fitness (log2 ratio) -1
0
1 at 12.622 kb on + strand, within GFF1628 at 12.622 kb on + strand, within GFF1628 at 12.773 kb on - strand, within GFF1628 at 12.849 kb on - strand, within GFF1628 at 13.103 kb on + strand, within GFF1628 at 13.103 kb on + strand, within GFF1628 at 13.146 kb on + strand at 13.181 kb on + strand at 13.261 kb on + strand at 13.626 kb on - strand, within GFF1629 at 13.626 kb on - strand, within GFF1629 at 13.659 kb on + strand, within GFF1629 at 13.660 kb on - strand, within GFF1629 at 13.681 kb on - strand, within GFF1629 at 13.861 kb on + strand, within GFF1629 at 13.861 kb on + strand, within GFF1629 at 13.940 kb on - strand at 13.940 kb on - strand at 14.031 kb on - strand at 14.031 kb on - strand at 14.150 kb on - strand at 14.333 kb on - strand, within GFF1630 at 14.390 kb on - strand, within GFF1630 at 14.593 kb on + strand, within GFF1630 at 14.594 kb on - strand, within GFF1630 at 14.594 kb on - strand, within GFF1630 at 14.741 kb on - strand, within GFF1630 at 14.741 kb on - strand, within GFF1630 at 14.845 kb on - strand, within GFF1630
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 12,622 + GFF1628 0.46 -0.2 12,622 + GFF1628 0.46 +1.0 12,773 - GFF1628 0.58 +0.2 12,849 - GFF1628 0.65 -0.1 13,103 + GFF1628 0.86 +0.4 13,103 + GFF1628 0.86 -0.9 13,146 + -0.2 13,181 + +0.7 13,261 + +0.3 13,626 - GFF1629 0.41 -0.7 13,626 - GFF1629 0.41 -0.2 13,659 + GFF1629 0.46 -0.3 13,660 - GFF1629 0.46 -1.2 13,681 - GFF1629 0.50 +0.1 13,861 + GFF1629 0.78 +0.1 13,861 + GFF1629 0.78 +0.1 13,940 - -0.4 13,940 - +0.9 14,031 - +0.4 14,031 - -0.4 14,150 - -0.2 14,333 - GFF1630 0.16 +0.5 14,390 - GFF1630 0.19 +0.2 14,593 + GFF1630 0.30 -0.7 14,594 - GFF1630 0.30 +0.8 14,594 - GFF1630 0.30 +0.2 14,741 - GFF1630 0.39 -0.1 14,741 - GFF1630 0.39 -0.4 14,845 - GFF1630 0.44 -0.6
Or see this region's nucleotide sequence