Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1557 and GFF1558 overlap by 14 nucleotides GFF1558 and GFF1559 are separated by 38 nucleotides GFF1559 and GFF1560 overlap by 4 nucleotides GFF1560 and GFF1561 are separated by 14 nucleotides GFF1561 and GFF1562 are separated by 10 nucleotides
GFF1557 - Tricarboxylate transport sensor protein TctE, at 20,541 to 21,956
GFF1557
GFF1558 - Tricarboxylate transport transcriptional regulator TctD, at 21,943 to 22,617
GFF1558
GFF1559 - hypothetical protein, at 22,656 to 22,775
GFF1559
GFF1560 - Tricarboxylate transport protein TctC, at 22,772 to 23,749
GFF1560
GFF1561 - Tricarboxylate transport protein TctB, at 23,764 to 24,195
GFF1561
GFF1562 - Tricarboxylate transport membrane protein TctA, at 24,206 to 25,720
GFF1562
Position (kb) 22
23
24 Strain fitness (log2 ratio) -1
0
1 at 21.818 kb on + strand at 21.860 kb on - strand at 21.928 kb on + strand at 22.042 kb on - strand, within GFF1558 at 22.203 kb on - strand, within GFF1558 at 22.325 kb on + strand, within GFF1558 at 22.458 kb on + strand, within GFF1558 at 22.712 kb on + strand, within GFF1559 at 22.823 kb on - strand at 22.967 kb on + strand, within GFF1560 at 22.967 kb on + strand, within GFF1560 at 22.968 kb on - strand, within GFF1560 at 22.968 kb on - strand, within GFF1560 at 22.969 kb on + strand, within GFF1560 at 22.970 kb on - strand, within GFF1560 at 23.248 kb on + strand, within GFF1560 at 23.543 kb on + strand, within GFF1560 at 23.544 kb on - strand, within GFF1560 at 23.574 kb on - strand, within GFF1560 at 23.688 kb on - strand at 23.705 kb on - strand at 23.851 kb on + strand, within GFF1561 at 23.852 kb on - strand, within GFF1561 at 23.978 kb on + strand, within GFF1561 at 24.007 kb on + strand, within GFF1561 at 24.007 kb on + strand, within GFF1561 at 24.139 kb on + strand, within GFF1561 at 24.171 kb on - strand at 24.171 kb on - strand at 24.394 kb on - strand, within GFF1562 at 24.413 kb on + strand, within GFF1562 at 24.413 kb on + strand, within GFF1562 at 24.414 kb on - strand, within GFF1562 at 24.414 kb on - strand, within GFF1562 at 24.429 kb on - strand, within GFF1562
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 21,818 + +0.7 21,860 - -0.7 21,928 + +0.3 22,042 - GFF1558 0.15 -0.6 22,203 - GFF1558 0.39 +1.6 22,325 + GFF1558 0.57 -1.0 22,458 + GFF1558 0.76 -0.3 22,712 + GFF1559 0.47 +0.5 22,823 - +0.4 22,967 + GFF1560 0.20 -0.4 22,967 + GFF1560 0.20 -0.2 22,968 - GFF1560 0.20 -0.2 22,968 - GFF1560 0.20 -0.4 22,969 + GFF1560 0.20 +0.3 22,970 - GFF1560 0.20 +0.5 23,248 + GFF1560 0.49 +0.1 23,543 + GFF1560 0.79 +0.3 23,544 - GFF1560 0.79 +0.5 23,574 - GFF1560 0.82 +1.7 23,688 - +0.2 23,705 - -0.6 23,851 + GFF1561 0.20 -0.3 23,852 - GFF1561 0.20 -0.4 23,978 + GFF1561 0.50 -0.6 24,007 + GFF1561 0.56 +0.0 24,007 + GFF1561 0.56 +1.5 24,139 + GFF1561 0.87 +0.4 24,171 - -0.1 24,171 - +1.2 24,394 - GFF1562 0.12 +0.5 24,413 + GFF1562 0.14 -0.0 24,413 + GFF1562 0.14 +0.6 24,414 - GFF1562 0.14 +0.2 24,414 - GFF1562 0.14 -0.2 24,429 - GFF1562 0.15 +1.4
Or see this region's nucleotide sequence