Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1302 and GFF1303 are separated by 177 nucleotides GFF1303 and GFF1304 are separated by 15 nucleotides GFF1304 and GFF1305 overlap by 16 nucleotides GFF1305 and GFF1306 overlap by 4 nucleotides
GFF1302 - Protease III precursor (EC 3.4.24.55), at 8,454 to 11,342
GFF1302
GFF1303 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 11,520 to 14,891
GFF1303
GFF1304 - FIG004136: Prepilin peptidase dependent protein C precursor, at 14,907 to 15,227
GFF1304
GFF1305 - FIG006270: hypothetical protein, at 15,212 to 15,619
GFF1305
GFF1306 - FIG004819: Prepilin peptidase dependent protein B precursor, at 15,616 to 16,140
GFF1306
Position (kb) 11
12
13
14
15 Strain fitness (log2 ratio) -1
0
1 at 10.572 kb on - strand, within GFF1302 at 10.629 kb on - strand, within GFF1302 at 10.658 kb on + strand, within GFF1302 at 10.917 kb on + strand, within GFF1302 at 11.010 kb on - strand, within GFF1302 at 11.010 kb on - strand, within GFF1302 at 11.066 kb on + strand at 11.066 kb on + strand at 11.066 kb on + strand at 11.067 kb on - strand at 11.244 kb on - strand at 11.359 kb on + strand at 11.392 kb on + strand at 11.420 kb on - strand at 11.422 kb on + strand at 11.423 kb on - strand at 11.456 kb on + strand at 13.639 kb on + strand, within GFF1303 at 13.640 kb on - strand, within GFF1303 at 14.071 kb on - strand, within GFF1303 at 15.099 kb on + strand, within GFF1304 at 15.120 kb on + strand, within GFF1304 at 15.162 kb on + strand, within GFF1304 at 15.400 kb on - strand, within GFF1305 at 15.434 kb on + strand, within GFF1305 at 15.434 kb on + strand, within GFF1305 at 15.435 kb on - strand, within GFF1305 at 15.485 kb on + strand, within GFF1305 at 15.520 kb on - strand, within GFF1305 at 15.540 kb on - strand, within GFF1305 at 15.552 kb on - strand, within GFF1305 at 15.572 kb on + strand, within GFF1305 at 15.587 kb on + strand at 15.591 kb on - strand at 15.741 kb on + strand, within GFF1306 at 15.757 kb on + strand, within GFF1306 at 15.882 kb on + strand, within GFF1306
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 10,572 - GFF1302 0.73 +0.3 10,629 - GFF1302 0.75 -1.6 10,658 + GFF1302 0.76 -0.2 10,917 + GFF1302 0.85 +0.9 11,010 - GFF1302 0.88 +0.1 11,010 - GFF1302 0.88 -0.3 11,066 + +1.1 11,066 + +0.0 11,066 + +0.1 11,067 - +0.6 11,244 - +0.3 11,359 + +0.3 11,392 + -0.6 11,420 - -0.4 11,422 + +0.0 11,423 - +0.9 11,456 + +0.6 13,639 + GFF1303 0.63 -0.9 13,640 - GFF1303 0.63 +0.1 14,071 - GFF1303 0.76 -1.5 15,099 + GFF1304 0.60 +0.4 15,120 + GFF1304 0.66 -0.5 15,162 + GFF1304 0.79 +0.5 15,400 - GFF1305 0.46 +0.1 15,434 + GFF1305 0.54 +0.2 15,434 + GFF1305 0.54 +0.1 15,435 - GFF1305 0.55 +0.4 15,485 + GFF1305 0.67 +0.9 15,520 - GFF1305 0.75 -1.5 15,540 - GFF1305 0.80 -0.9 15,552 - GFF1305 0.83 -1.0 15,572 + GFF1305 0.88 +0.5 15,587 + -0.1 15,591 - +1.1 15,741 + GFF1306 0.24 +0.0 15,757 + GFF1306 0.27 -0.4 15,882 + GFF1306 0.51 -0.1
Or see this region's nucleotide sequence