Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1301 and GFF1302 overlap by 8 nucleotides GFF1302 and GFF1303 are separated by 177 nucleotides
GFF1301 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 4,916 to 8,461
GFF1301
GFF1302 - Protease III precursor (EC 3.4.24.55), at 8,454 to 11,342
GFF1302
GFF1303 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 11,520 to 14,891
GFF1303
Position (kb)
8
9
10
11
12 Strain fitness (log2 ratio)
-1
0
1 at 8.539 kb on + strand at 8.540 kb on - strand at 8.540 kb on - strand at 8.716 kb on - strand at 9.006 kb on + strand, within GFF1302 at 9.006 kb on + strand, within GFF1302 at 9.007 kb on - strand, within GFF1302 at 9.087 kb on - strand, within GFF1302 at 9.087 kb on - strand, within GFF1302 at 9.222 kb on - strand, within GFF1302 at 9.269 kb on + strand, within GFF1302 at 9.270 kb on - strand, within GFF1302 at 9.338 kb on - strand, within GFF1302 at 9.338 kb on - strand, within GFF1302 at 9.369 kb on - strand, within GFF1302 at 9.369 kb on - strand, within GFF1302 at 9.467 kb on + strand, within GFF1302 at 9.515 kb on + strand, within GFF1302 at 9.516 kb on - strand, within GFF1302 at 9.558 kb on - strand, within GFF1302 at 9.622 kb on + strand, within GFF1302 at 9.622 kb on + strand, within GFF1302 at 9.721 kb on + strand, within GFF1302 at 9.944 kb on + strand, within GFF1302 at 10.112 kb on - strand, within GFF1302 at 10.181 kb on - strand, within GFF1302 at 10.188 kb on - strand, within GFF1302 at 10.337 kb on + strand, within GFF1302 at 10.401 kb on + strand, within GFF1302 at 10.405 kb on + strand, within GFF1302 at 10.482 kb on - strand, within GFF1302 at 10.572 kb on - strand, within GFF1302 at 10.629 kb on - strand, within GFF1302 at 10.658 kb on + strand, within GFF1302 at 10.917 kb on + strand, within GFF1302 at 11.010 kb on - strand, within GFF1302 at 11.010 kb on - strand, within GFF1302 at 11.066 kb on + strand at 11.066 kb on + strand at 11.066 kb on + strand at 11.067 kb on - strand at 11.244 kb on - strand at 11.359 kb on + strand at 11.392 kb on + strand at 11.420 kb on - strand at 11.422 kb on + strand at 11.423 kb on - strand at 11.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 8,539 + +0.4 8,540 - +0.8 8,540 - +0.0 8,716 - -0.3 9,006 + GFF1302 0.19 -0.2 9,006 + GFF1302 0.19 +0.2 9,007 - GFF1302 0.19 +0.5 9,087 - GFF1302 0.22 +0.8 9,087 - GFF1302 0.22 +1.1 9,222 - GFF1302 0.27 +0.5 9,269 + GFF1302 0.28 -1.0 9,270 - GFF1302 0.28 +0.1 9,338 - GFF1302 0.31 +0.5 9,338 - GFF1302 0.31 +0.0 9,369 - GFF1302 0.32 +0.1 9,369 - GFF1302 0.32 +0.7 9,467 + GFF1302 0.35 +0.1 9,515 + GFF1302 0.37 -0.3 9,516 - GFF1302 0.37 +0.3 9,558 - GFF1302 0.38 +0.3 9,622 + GFF1302 0.40 -0.2 9,622 + GFF1302 0.40 -0.1 9,721 + GFF1302 0.44 +0.5 9,944 + GFF1302 0.52 -0.1 10,112 - GFF1302 0.57 +0.3 10,181 - GFF1302 0.60 +0.6 10,188 - GFF1302 0.60 +0.1 10,337 + GFF1302 0.65 +0.2 10,401 + GFF1302 0.67 +0.7 10,405 + GFF1302 0.68 +0.2 10,482 - GFF1302 0.70 -0.4 10,572 - GFF1302 0.73 +0.3 10,629 - GFF1302 0.75 -1.6 10,658 + GFF1302 0.76 -0.2 10,917 + GFF1302 0.85 +0.9 11,010 - GFF1302 0.88 +0.1 11,010 - GFF1302 0.88 -0.3 11,066 + +1.1 11,066 + +0.0 11,066 + +0.1 11,067 - +0.6 11,244 - +0.3 11,359 + +0.3 11,392 + -0.6 11,420 - -0.4 11,422 + +0.0 11,423 - +0.9 11,456 + +0.6
Or see this region's nucleotide sequence