Experiment: LB_plus_SM_buffer with Ffm_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1012 and GFF1013 are separated by 14 nucleotides GFF1013 and GFF1014 are separated by 25 nucleotides GFF1014 and GFF1015 overlap by 23 nucleotides GFF1015 and GFF1016 are separated by 12 nucleotides
GFF1012 - PTS system, mannose-specific IIA component (EC 2.7.1.69), at 198,286 to 198,708
GFF1012
GFF1013 - PTS system, mannose-specific IIB component (EC 2.7.1.69), at 198,723 to 199,184
GFF1013
GFF1014 - PTS system, mannose-specific IIC component (EC 2.7.1.69), at 199,210 to 199,989
GFF1014
GFF1015 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 199,967 to 200,815
GFF1015
GFF1016 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 200,828 to 201,889
GFF1016
Position (kb) 199
200 Strain fitness (log2 ratio) -1
0
1 at 198.359 kb on + strand, within GFF1012 at 198.438 kb on - strand, within GFF1012 at 198.615 kb on + strand, within GFF1012 at 198.806 kb on + strand, within GFF1013 at 198.833 kb on + strand, within GFF1013 at 199.206 kb on + strand at 199.207 kb on - strand at 199.210 kb on - strand at 199.210 kb on - strand at 199.531 kb on - strand, within GFF1014 at 200.492 kb on + strand, within GFF1015 at 200.493 kb on - strand, within GFF1015 at 200.629 kb on + strand, within GFF1015 at 200.671 kb on + strand, within GFF1015 at 200.849 kb on + strand at 200.850 kb on - strand at 200.958 kb on + strand, within GFF1016
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 15 MOI remove 198,359 + GFF1012 0.17 +0.2 198,438 - GFF1012 0.36 +1.2 198,615 + GFF1012 0.78 +0.1 198,806 + GFF1013 0.18 +0.5 198,833 + GFF1013 0.24 +0.4 199,206 + +0.9 199,207 - -0.1 199,210 - +0.2 199,210 - -0.8 199,531 - GFF1014 0.41 -0.6 200,492 + GFF1015 0.62 -0.2 200,493 - GFF1015 0.62 -0.5 200,629 + GFF1015 0.78 +0.8 200,671 + GFF1015 0.83 -0.6 200,849 + +0.4 200,850 - +0.2 200,958 + GFF1016 0.12 -0.1
Or see this region's nucleotide sequence