Experiment: Cholinium glutamate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5583 and PP_3110 overlap by 41 nucleotides PP_3110 and PP_5584 are separated by 8 nucleotides PP_5584 and PP_3113 are separated by 696 nucleotides
PP_5583: PP_5583 - conserved protein of unknown function, at 3,521,488 to 3,521,847
_5583
PP_3110: PP_3110 - protein of unknown function, at 3,521,807 to 3,522,019
_3110
PP_5584: PP_5584 - protein of unknown function, at 3,522,028 to 3,522,213
_5584
PP_3113: PP_3113 - transposase, at 3,522,910 to 3,523,269
_3113
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-1
0
1
2 at 3520.829 kb on + strand at 3520.830 kb on - strand at 3520.982 kb on + strand at 3520.983 kb on - strand at 3520.983 kb on - strand at 3521.051 kb on + strand at 3521.133 kb on + strand at 3521.140 kb on - strand at 3521.369 kb on + strand at 3521.370 kb on - strand at 3521.671 kb on - strand, within PP_5583 at 3521.799 kb on + strand, within PP_5583 at 3521.931 kb on - strand, within PP_3110 at 3521.987 kb on + strand, within PP_3110 at 3521.988 kb on - strand, within PP_3110 at 3522.284 kb on + strand at 3522.284 kb on + strand at 3522.284 kb on + strand at 3522.345 kb on - strand at 3522.428 kb on + strand at 3522.428 kb on + strand at 3522.428 kb on + strand at 3522.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium glutamate 10 vol% remove 3,520,829 + -0.2 3,520,830 - +1.5 3,520,982 + -1.2 3,520,983 - -0.6 3,520,983 - -0.1 3,521,051 + -0.8 3,521,133 + +0.5 3,521,140 - -0.5 3,521,369 + +2.3 3,521,370 - -0.0 3,521,671 - PP_5583 0.51 +0.8 3,521,799 + PP_5583 0.86 -0.1 3,521,931 - PP_3110 0.58 -0.5 3,521,987 + PP_3110 0.85 -0.1 3,521,988 - PP_3110 0.85 -0.5 3,522,284 + +0.5 3,522,284 + -0.2 3,522,284 + -1.5 3,522,345 - +0.0 3,522,428 + -0.5 3,522,428 + +0.3 3,522,428 + -0.7 3,522,537 - -0.4
Or see this region's nucleotide sequence