Experiment: Cholinium glutamate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0006 and PP_0007 are separated by 2 nucleotides PP_0007 and PP_0008 overlap by 8 nucleotides PP_0008 and PP_0009 are separated by 13 nucleotides
PP_0006: PP_0006 - probable membrane protein insertase, at 6,471 to 8,153
_0006
PP_0007: PP_0007 - conserved hypothetical protein, at 8,156 to 8,401
_0007
PP_0008: PP_0008 - ribonuclease P protein component, at 8,394 to 8,798
_0008
PP_0009: PP_0009 - 50S ribosomal protein L34, at 8,812 to 8,946
_0009
Position (kb) 8
9 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 8.209 kb on - strand, within PP_0007 at 8.209 kb on - strand, within PP_0007 at 8.299 kb on - strand at 8.299 kb on - strand, within PP_0007 at 8.354 kb on - strand, within PP_0007 at 8.396 kb on - strand at 9.056 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.152 kb on + strand at 9.154 kb on + strand at 9.154 kb on + strand at 9.155 kb on - strand at 9.177 kb on - strand at 9.186 kb on - strand at 9.238 kb on + strand at 9.238 kb on + strand at 9.249 kb on - strand at 9.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium glutamate 10 vol% remove 8,209 - PP_0007 0.22 -2.9 8,209 - PP_0007 0.22 +0.0 8,299 - -0.9 8,299 - PP_0007 0.58 -1.5 8,354 - PP_0007 0.80 -2.1 8,396 - -0.9 9,056 - -0.6 9,058 - -0.3 9,058 - -0.3 9,058 - -0.7 9,058 - -0.5 9,152 + +0.9 9,154 + -0.1 9,154 + +0.3 9,155 - +0.6 9,177 - -0.5 9,186 - -2.1 9,238 + +0.3 9,238 + +2.2 9,249 - +0.1 9,395 - -1.1
Or see this region's nucleotide sequence