Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1145 and PfGW456L13_1146 are separated by 63 nucleotides PfGW456L13_1146 and PfGW456L13_1147 are separated by 93 nucleotides PfGW456L13_1147 and PfGW456L13_1148 are separated by 174 nucleotides
PfGW456L13_1145 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 1,273,765 to 1,274,529
_1145
PfGW456L13_1146 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 1,274,593 to 1,275,333
_1146
PfGW456L13_1147 - Biotin synthase (EC 2.8.1.6), at 1,275,427 to 1,276,482
_1147
PfGW456L13_1148 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 1,276,657 to 1,277,835
_1148
Position (kb)
1275
1276
1277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1274.633 kb on + strand at 1274.634 kb on - strand at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.707 kb on + strand, within PfGW456L13_1146 at 1274.708 kb on - strand, within PfGW456L13_1146 at 1274.708 kb on - strand, within PfGW456L13_1146 at 1274.708 kb on - strand, within PfGW456L13_1146 at 1274.708 kb on - strand, within PfGW456L13_1146 at 1274.999 kb on + strand, within PfGW456L13_1146 at 1275.000 kb on - strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.089 kb on + strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.090 kb on - strand, within PfGW456L13_1146 at 1275.344 kb on + strand at 1275.344 kb on + strand at 1275.344 kb on + strand at 1275.344 kb on + strand at 1275.344 kb on + strand at 1275.344 kb on + strand at 1275.344 kb on + strand at 1275.345 kb on - strand at 1275.345 kb on - strand at 1275.345 kb on - strand at 1275.348 kb on + strand at 1275.349 kb on - strand at 1275.349 kb on - strand at 1275.349 kb on - strand at 1275.349 kb on - strand at 1275.408 kb on + strand at 1275.408 kb on + strand at 1275.408 kb on + strand at 1275.408 kb on + strand at 1275.408 kb on + strand at 1275.408 kb on + strand at 1275.408 kb on + strand at 1275.409 kb on - strand at 1275.409 kb on - strand at 1275.409 kb on - strand at 1275.409 kb on - strand at 1275.409 kb on - strand at 1275.409 kb on - strand at 1275.409 kb on - strand at 1275.619 kb on + strand, within PfGW456L13_1147 at 1275.619 kb on + strand, within PfGW456L13_1147 at 1275.619 kb on + strand, within PfGW456L13_1147 at 1275.619 kb on + strand, within PfGW456L13_1147 at 1275.620 kb on - strand, within PfGW456L13_1147 at 1275.620 kb on - strand, within PfGW456L13_1147 at 1275.620 kb on - strand, within PfGW456L13_1147 at 1275.620 kb on - strand, within PfGW456L13_1147 at 1275.640 kb on + strand, within PfGW456L13_1147 at 1275.640 kb on + strand, within PfGW456L13_1147 at 1275.640 kb on + strand, within PfGW456L13_1147 at 1275.640 kb on + strand, within PfGW456L13_1147 at 1275.641 kb on - strand, within PfGW456L13_1147 at 1275.641 kb on - strand, within PfGW456L13_1147 at 1275.641 kb on - strand, within PfGW456L13_1147 at 1275.641 kb on - strand, within PfGW456L13_1147 at 1275.779 kb on - strand, within PfGW456L13_1147 at 1275.779 kb on - strand, within PfGW456L13_1147 at 1275.889 kb on + strand, within PfGW456L13_1147 at 1275.889 kb on + strand, within PfGW456L13_1147 at 1275.889 kb on + strand, within PfGW456L13_1147 at 1275.890 kb on - strand, within PfGW456L13_1147 at 1275.890 kb on - strand, within PfGW456L13_1147 at 1275.890 kb on - strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.892 kb on + strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.893 kb on - strand, within PfGW456L13_1147 at 1275.909 kb on + strand, within PfGW456L13_1147 at 1275.909 kb on + strand, within PfGW456L13_1147 at 1275.925 kb on + strand, within PfGW456L13_1147 at 1275.926 kb on - strand, within PfGW456L13_1147 at 1275.926 kb on - strand, within PfGW456L13_1147 at 1275.952 kb on + strand, within PfGW456L13_1147 at 1275.952 kb on + strand, within PfGW456L13_1147 at 1275.953 kb on - strand, within PfGW456L13_1147 at 1275.953 kb on - strand, within PfGW456L13_1147 at 1275.953 kb on - strand, within PfGW456L13_1147 at 1275.979 kb on + strand, within PfGW456L13_1147 at 1275.980 kb on - strand, within PfGW456L13_1147 at 1275.980 kb on - strand, within PfGW456L13_1147 at 1275.980 kb on - strand, within PfGW456L13_1147 at 1275.980 kb on - strand, within PfGW456L13_1147 at 1276.422 kb on + strand at 1276.423 kb on - strand at 1276.423 kb on - strand at 1276.423 kb on - strand at 1276.465 kb on + strand at 1276.465 kb on + strand at 1276.786 kb on - strand, within PfGW456L13_1148 at 1276.795 kb on + strand, within PfGW456L13_1148 at 1276.795 kb on + strand, within PfGW456L13_1148 at 1276.796 kb on - strand, within PfGW456L13_1148 at 1276.796 kb on - strand, within PfGW456L13_1148 at 1276.896 kb on + strand, within PfGW456L13_1148 at 1276.896 kb on + strand, within PfGW456L13_1148 at 1276.896 kb on + strand, within PfGW456L13_1148 at 1276.896 kb on + strand, within PfGW456L13_1148 at 1276.896 kb on + strand, within PfGW456L13_1148 at 1276.896 kb on + strand, within PfGW456L13_1148 at 1276.897 kb on - strand, within PfGW456L13_1148 at 1276.897 kb on - strand, within PfGW456L13_1148 at 1276.897 kb on - strand, within PfGW456L13_1148 at 1276.897 kb on - strand, within PfGW456L13_1148 at 1276.897 kb on - strand, within PfGW456L13_1148 at 1276.975 kb on + strand, within PfGW456L13_1148 at 1276.976 kb on - strand, within PfGW456L13_1148 at 1276.976 kb on - strand, within PfGW456L13_1148 at 1276.976 kb on - strand, within PfGW456L13_1148 at 1276.976 kb on - strand, within PfGW456L13_1148 at 1276.976 kb on - strand, within PfGW456L13_1148 at 1277.022 kb on + strand, within PfGW456L13_1148 at 1277.022 kb on + strand, within PfGW456L13_1148 at 1277.098 kb on + strand, within PfGW456L13_1148 at 1277.099 kb on - strand, within PfGW456L13_1148 at 1277.154 kb on + strand, within PfGW456L13_1148 at 1277.154 kb on + strand, within PfGW456L13_1148 at 1277.154 kb on + strand, within PfGW456L13_1148 at 1277.154 kb on + strand, within PfGW456L13_1148 at 1277.154 kb on + strand, within PfGW456L13_1148 at 1277.155 kb on - strand, within PfGW456L13_1148 at 1277.155 kb on - strand, within PfGW456L13_1148 at 1277.440 kb on + strand, within PfGW456L13_1148 at 1277.440 kb on + strand, within PfGW456L13_1148 at 1277.440 kb on + strand, within PfGW456L13_1148 at 1277.440 kb on + strand, within PfGW456L13_1148 at 1277.441 kb on - strand, within PfGW456L13_1148 at 1277.441 kb on - strand, within PfGW456L13_1148 at 1277.441 kb on - strand, within PfGW456L13_1148 at 1277.441 kb on - strand, within PfGW456L13_1148 at 1277.441 kb on - strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.446 kb on + strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148 at 1277.447 kb on - strand, within PfGW456L13_1148
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 1,274,633 + +1.0 1,274,634 - +0.3 1,274,707 + PfGW456L13_1146 0.15 -0.1 1,274,707 + PfGW456L13_1146 0.15 +2.9 1,274,707 + PfGW456L13_1146 0.15 -0.2 1,274,707 + PfGW456L13_1146 0.15 +0.2 1,274,707 + PfGW456L13_1146 0.15 +0.4 1,274,707 + PfGW456L13_1146 0.15 -2.2 1,274,707 + PfGW456L13_1146 0.15 -0.1 1,274,707 + PfGW456L13_1146 0.15 -0.1 1,274,707 + PfGW456L13_1146 0.15 -0.5 1,274,708 - PfGW456L13_1146 0.16 +0.6 1,274,708 - PfGW456L13_1146 0.16 +0.2 1,274,708 - PfGW456L13_1146 0.16 +0.8 1,274,708 - PfGW456L13_1146 0.16 -0.5 1,274,999 + PfGW456L13_1146 0.55 +0.7 1,275,000 - PfGW456L13_1146 0.55 -1.2 1,275,089 + PfGW456L13_1146 0.67 -0.5 1,275,089 + PfGW456L13_1146 0.67 +0.8 1,275,089 + PfGW456L13_1146 0.67 -0.1 1,275,089 + PfGW456L13_1146 0.67 -0.6 1,275,089 + PfGW456L13_1146 0.67 +0.2 1,275,089 + PfGW456L13_1146 0.67 -0.7 1,275,089 + PfGW456L13_1146 0.67 +0.7 1,275,089 + PfGW456L13_1146 0.67 -0.3 1,275,089 + PfGW456L13_1146 0.67 -0.1 1,275,090 - PfGW456L13_1146 0.67 +0.1 1,275,090 - PfGW456L13_1146 0.67 -0.1 1,275,090 - PfGW456L13_1146 0.67 +0.3 1,275,090 - PfGW456L13_1146 0.67 +0.0 1,275,090 - PfGW456L13_1146 0.67 -0.3 1,275,090 - PfGW456L13_1146 0.67 -0.3 1,275,090 - PfGW456L13_1146 0.67 -0.3 1,275,344 + +0.3 1,275,344 + +0.6 1,275,344 + -1.3 1,275,344 + -0.8 1,275,344 + +0.3 1,275,344 + +0.7 1,275,344 + +0.2 1,275,345 - -2.0 1,275,345 - -1.3 1,275,345 - -0.9 1,275,348 + -0.3 1,275,349 - -2.1 1,275,349 - -0.8 1,275,349 - -1.5 1,275,349 - -1.6 1,275,408 + +0.2 1,275,408 + -0.4 1,275,408 + +0.5 1,275,408 + -0.3 1,275,408 + -0.1 1,275,408 + +0.8 1,275,408 + +0.2 1,275,409 - -1.2 1,275,409 - -1.6 1,275,409 - -0.8 1,275,409 - -1.7 1,275,409 - +0.1 1,275,409 - -0.6 1,275,409 - -2.3 1,275,619 + PfGW456L13_1147 0.18 -1.8 1,275,619 + PfGW456L13_1147 0.18 -3.0 1,275,619 + PfGW456L13_1147 0.18 -2.2 1,275,619 + PfGW456L13_1147 0.18 -2.1 1,275,620 - PfGW456L13_1147 0.18 -1.3 1,275,620 - PfGW456L13_1147 0.18 -0.7 1,275,620 - PfGW456L13_1147 0.18 -1.9 1,275,620 - PfGW456L13_1147 0.18 -1.4 1,275,640 + PfGW456L13_1147 0.20 -1.8 1,275,640 + PfGW456L13_1147 0.20 +0.4 1,275,640 + PfGW456L13_1147 0.20 -2.1 1,275,640 + PfGW456L13_1147 0.20 -1.0 1,275,641 - PfGW456L13_1147 0.20 -0.5 1,275,641 - PfGW456L13_1147 0.20 -0.7 1,275,641 - PfGW456L13_1147 0.20 -2.1 1,275,641 - PfGW456L13_1147 0.20 -1.9 1,275,779 - PfGW456L13_1147 0.33 -2.6 1,275,779 - PfGW456L13_1147 0.33 -0.8 1,275,889 + PfGW456L13_1147 0.44 -1.3 1,275,889 + PfGW456L13_1147 0.44 -1.2 1,275,889 + PfGW456L13_1147 0.44 -1.7 1,275,890 - PfGW456L13_1147 0.44 -1.9 1,275,890 - PfGW456L13_1147 0.44 -1.9 1,275,890 - PfGW456L13_1147 0.44 -1.0 1,275,892 + PfGW456L13_1147 0.44 -0.6 1,275,892 + PfGW456L13_1147 0.44 -0.0 1,275,892 + PfGW456L13_1147 0.44 -1.6 1,275,892 + PfGW456L13_1147 0.44 -1.9 1,275,892 + PfGW456L13_1147 0.44 -2.7 1,275,892 + PfGW456L13_1147 0.44 -1.0 1,275,892 + PfGW456L13_1147 0.44 -1.7 1,275,892 + PfGW456L13_1147 0.44 -0.7 1,275,892 + PfGW456L13_1147 0.44 -1.4 1,275,892 + PfGW456L13_1147 0.44 -2.5 1,275,892 + PfGW456L13_1147 0.44 -2.0 1,275,892 + PfGW456L13_1147 0.44 -1.0 1,275,892 + PfGW456L13_1147 0.44 -2.6 1,275,892 + PfGW456L13_1147 0.44 -1.6 1,275,892 + PfGW456L13_1147 0.44 -1.0 1,275,892 + PfGW456L13_1147 0.44 -1.3 1,275,893 - PfGW456L13_1147 0.44 +0.7 1,275,893 - PfGW456L13_1147 0.44 -0.8 1,275,893 - PfGW456L13_1147 0.44 -2.5 1,275,893 - PfGW456L13_1147 0.44 -2.2 1,275,893 - PfGW456L13_1147 0.44 -3.3 1,275,893 - PfGW456L13_1147 0.44 -1.4 1,275,893 - PfGW456L13_1147 0.44 -1.6 1,275,909 + PfGW456L13_1147 0.46 -0.9 1,275,909 + PfGW456L13_1147 0.46 -1.4 1,275,925 + PfGW456L13_1147 0.47 -1.0 1,275,926 - PfGW456L13_1147 0.47 -1.4 1,275,926 - PfGW456L13_1147 0.47 -0.6 1,275,952 + PfGW456L13_1147 0.50 -1.3 1,275,952 + PfGW456L13_1147 0.50 -1.1 1,275,953 - PfGW456L13_1147 0.50 +0.7 1,275,953 - PfGW456L13_1147 0.50 -1.8 1,275,953 - PfGW456L13_1147 0.50 -0.5 1,275,979 + PfGW456L13_1147 0.52 -0.0 1,275,980 - PfGW456L13_1147 0.52 -0.4 1,275,980 - PfGW456L13_1147 0.52 -1.1 1,275,980 - PfGW456L13_1147 0.52 -1.1 1,275,980 - PfGW456L13_1147 0.52 -0.4 1,276,422 + -1.7 1,276,423 - -0.5 1,276,423 - -0.0 1,276,423 - -0.3 1,276,465 + +0.9 1,276,465 + -0.6 1,276,786 - PfGW456L13_1148 0.11 +0.0 1,276,795 + PfGW456L13_1148 0.12 -1.1 1,276,795 + PfGW456L13_1148 0.12 -1.3 1,276,796 - PfGW456L13_1148 0.12 -0.3 1,276,796 - PfGW456L13_1148 0.12 -0.7 1,276,896 + PfGW456L13_1148 0.20 +0.2 1,276,896 + PfGW456L13_1148 0.20 -1.2 1,276,896 + PfGW456L13_1148 0.20 +0.4 1,276,896 + PfGW456L13_1148 0.20 -0.2 1,276,896 + PfGW456L13_1148 0.20 -0.3 1,276,896 + PfGW456L13_1148 0.20 -0.8 1,276,897 - PfGW456L13_1148 0.20 +0.1 1,276,897 - PfGW456L13_1148 0.20 -0.2 1,276,897 - PfGW456L13_1148 0.20 -0.5 1,276,897 - PfGW456L13_1148 0.20 -2.4 1,276,897 - PfGW456L13_1148 0.20 -0.2 1,276,975 + PfGW456L13_1148 0.27 +0.5 1,276,976 - PfGW456L13_1148 0.27 -0.6 1,276,976 - PfGW456L13_1148 0.27 -1.0 1,276,976 - PfGW456L13_1148 0.27 +0.3 1,276,976 - PfGW456L13_1148 0.27 -0.3 1,276,976 - PfGW456L13_1148 0.27 -1.6 1,277,022 + PfGW456L13_1148 0.31 +0.1 1,277,022 + PfGW456L13_1148 0.31 -0.2 1,277,098 + PfGW456L13_1148 0.37 +0.5 1,277,099 - PfGW456L13_1148 0.37 +0.1 1,277,154 + PfGW456L13_1148 0.42 -0.9 1,277,154 + PfGW456L13_1148 0.42 +0.0 1,277,154 + PfGW456L13_1148 0.42 +0.7 1,277,154 + PfGW456L13_1148 0.42 -0.3 1,277,154 + PfGW456L13_1148 0.42 -0.1 1,277,155 - PfGW456L13_1148 0.42 -0.3 1,277,155 - PfGW456L13_1148 0.42 -0.8 1,277,440 + PfGW456L13_1148 0.66 -1.2 1,277,440 + PfGW456L13_1148 0.66 -0.4 1,277,440 + PfGW456L13_1148 0.66 -0.9 1,277,440 + PfGW456L13_1148 0.66 -0.2 1,277,441 - PfGW456L13_1148 0.66 -0.9 1,277,441 - PfGW456L13_1148 0.66 -1.3 1,277,441 - PfGW456L13_1148 0.66 -0.6 1,277,441 - PfGW456L13_1148 0.66 -1.4 1,277,441 - PfGW456L13_1148 0.66 +0.2 1,277,446 + PfGW456L13_1148 0.67 -0.8 1,277,446 + PfGW456L13_1148 0.67 +1.4 1,277,446 + PfGW456L13_1148 0.67 -0.3 1,277,446 + PfGW456L13_1148 0.67 -0.7 1,277,446 + PfGW456L13_1148 0.67 -0.9 1,277,446 + PfGW456L13_1148 0.67 -0.6 1,277,446 + PfGW456L13_1148 0.67 -0.0 1,277,446 + PfGW456L13_1148 0.67 -0.3 1,277,446 + PfGW456L13_1148 0.67 +0.1 1,277,446 + PfGW456L13_1148 0.67 -0.4 1,277,446 + PfGW456L13_1148 0.67 +0.1 1,277,447 - PfGW456L13_1148 0.67 -0.6 1,277,447 - PfGW456L13_1148 0.67 +0.4 1,277,447 - PfGW456L13_1148 0.67 -0.3 1,277,447 - PfGW456L13_1148 0.67 -0.8 1,277,447 - PfGW456L13_1148 0.67 +0.2 1,277,447 - PfGW456L13_1148 0.67 +0.1 1,277,447 - PfGW456L13_1148 0.67 -0.6 1,277,447 - PfGW456L13_1148 0.67 -0.7 1,277,447 - PfGW456L13_1148 0.67 -0.3 1,277,447 - PfGW456L13_1148 0.67 -0.2 1,277,447 - PfGW456L13_1148 0.67 -0.3 1,277,447 - PfGW456L13_1148 0.67 -0.2 1,277,447 - PfGW456L13_1148 0.67 -0.9 1,277,447 - PfGW456L13_1148 0.67 -0.2 1,277,447 - PfGW456L13_1148 0.67 -1.0 1,277,447 - PfGW456L13_1148 0.67 -1.5 1,277,447 - PfGW456L13_1148 0.67 -0.6 1,277,447 - PfGW456L13_1148 0.67 -1.2
Or see this region's nucleotide sequence