Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4789 and GFF4790 are separated by 13 nucleotides GFF4790 and GFF4791 are separated by 138 nucleotides GFF4791 and GFF4792 overlap by 7 nucleotides GFF4792 and GFF4793 overlap by 11 nucleotides
GFF4789 - Lipoprotein NlpD, at 96,332 to 97,291
GFF4789
GFF4790 - FIG01047833: hypothetical protein, at 97,305 to 97,502
GFF4790
GFF4791 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 97,641 to 98,267
GFF4791
GFF4792 - 5-nucleotidase SurE (EC 3.1.3.5) @ Exopolyphosphatase (EC 3.6.1.11), at 98,261 to 99,013
GFF4792
GFF4793 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 99,003 to 100,052
GFF4793
Position (kb) 98
99
100 Strain fitness (log2 ratio) -1
0
1
2 at 97.386 kb on + strand, within GFF4790 at 97.387 kb on + strand, within GFF4790 at 97.524 kb on + strand at 97.617 kb on - strand at 97.617 kb on - strand at 97.643 kb on - strand at 97.663 kb on - strand at 97.802 kb on + strand, within GFF4791 at 97.902 kb on + strand, within GFF4791 at 97.903 kb on - strand, within GFF4791 at 98.081 kb on + strand, within GFF4791 at 98.082 kb on - strand, within GFF4791 at 98.083 kb on + strand, within GFF4791 at 98.083 kb on + strand, within GFF4791 at 98.084 kb on - strand, within GFF4791 at 98.084 kb on - strand, within GFF4791 at 98.127 kb on - strand, within GFF4791 at 98.142 kb on - strand, within GFF4791 at 98.162 kb on + strand, within GFF4791 at 98.250 kb on + strand at 98.251 kb on - strand at 98.407 kb on - strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.441 kb on - strand, within GFF4792 at 98.469 kb on - strand, within GFF4792 at 98.535 kb on - strand, within GFF4792 at 98.535 kb on - strand, within GFF4792 at 98.631 kb on + strand, within GFF4792 at 98.631 kb on + strand, within GFF4792 at 98.787 kb on - strand, within GFF4792 at 98.835 kb on - strand, within GFF4792 at 99.015 kb on - strand at 99.094 kb on - strand at 99.129 kb on + strand, within GFF4793 at 99.130 kb on - strand, within GFF4793 at 99.287 kb on + strand, within GFF4793 at 99.660 kb on - strand, within GFF4793 at 99.695 kb on + strand, within GFF4793 at 99.695 kb on + strand, within GFF4793 at 99.696 kb on - strand, within GFF4793 at 99.713 kb on - strand, within GFF4793
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 97,386 + GFF4790 0.41 -0.0 97,387 + GFF4790 0.41 -0.4 97,524 + -0.1 97,617 - -0.8 97,617 - +0.4 97,643 - -0.2 97,663 - +0.1 97,802 + GFF4791 0.26 -0.5 97,902 + GFF4791 0.42 -0.8 97,903 - GFF4791 0.42 -0.2 98,081 + GFF4791 0.70 +0.8 98,082 - GFF4791 0.70 -0.6 98,083 + GFF4791 0.70 +0.6 98,083 + GFF4791 0.70 -1.0 98,084 - GFF4791 0.71 -0.2 98,084 - GFF4791 0.71 +1.8 98,127 - GFF4791 0.78 -1.1 98,142 - GFF4791 0.80 +0.7 98,162 + GFF4791 0.83 -0.1 98,250 + -0.4 98,251 - +0.4 98,407 - GFF4792 0.19 -1.3 98,440 + GFF4792 0.24 -1.0 98,440 + GFF4792 0.24 -0.3 98,440 + GFF4792 0.24 -0.2 98,440 + GFF4792 0.24 -1.2 98,440 + GFF4792 0.24 -0.8 98,441 - GFF4792 0.24 -0.9 98,469 - GFF4792 0.28 -0.6 98,535 - GFF4792 0.36 -1.3 98,535 - GFF4792 0.36 -0.4 98,631 + GFF4792 0.49 -0.6 98,631 + GFF4792 0.49 +0.2 98,787 - GFF4792 0.70 -0.1 98,835 - GFF4792 0.76 -0.4 99,015 - +0.1 99,094 - -0.1 99,129 + GFF4793 0.12 -0.6 99,130 - GFF4793 0.12 -0.8 99,287 + GFF4793 0.27 -0.9 99,660 - GFF4793 0.63 +0.5 99,695 + GFF4793 0.66 +0.6 99,695 + GFF4793 0.66 +0.8 99,696 - GFF4793 0.66 +0.2 99,713 - GFF4793 0.68 +0.7
Or see this region's nucleotide sequence