Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4601 and GFF4602 are separated by 114 nucleotides GFF4602 and GFF4603 are separated by 46 nucleotides GFF4603 and GFF4604 overlap by 4 nucleotides
GFF4601 - Phosphoglucomutase (EC 5.4.2.2), at 390,302 to 391,942
GFF4601
GFF4602 - 'Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)' transl_table=11, at 392,057 to 392,560
GFF4602
GFF4603 - Putrescine/proton symporter, putrescine/ornithine antiporter PotE, at 392,607 to 393,926
GFF4603
GFF4604 - Ornithine decarboxylase (EC 4.1.1.17), at 393,923 to 396,121
GFF4604
Position (kb)
392
393
394 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 391.634 kb on + strand, within GFF4601 at 392.137 kb on + strand, within GFF4602 at 392.137 kb on + strand, within GFF4602 at 392.138 kb on - strand, within GFF4602 at 392.138 kb on - strand, within GFF4602 at 392.266 kb on - strand, within GFF4602 at 392.270 kb on - strand, within GFF4602 at 392.270 kb on - strand, within GFF4602 at 392.271 kb on + strand, within GFF4602 at 392.294 kb on + strand, within GFF4602 at 392.295 kb on - strand, within GFF4602 at 392.295 kb on - strand, within GFF4602 at 392.295 kb on - strand, within GFF4602 at 392.298 kb on - strand, within GFF4602 at 392.346 kb on - strand, within GFF4602 at 392.513 kb on - strand at 392.581 kb on - strand at 392.654 kb on - strand at 392.656 kb on - strand at 392.732 kb on + strand at 392.734 kb on + strand at 392.734 kb on + strand at 392.735 kb on - strand at 392.836 kb on + strand, within GFF4603 at 392.843 kb on + strand, within GFF4603 at 392.843 kb on + strand, within GFF4603 at 392.844 kb on - strand, within GFF4603 at 393.215 kb on - strand, within GFF4603 at 393.215 kb on - strand, within GFF4603 at 393.327 kb on + strand, within GFF4603 at 393.393 kb on + strand, within GFF4603 at 393.397 kb on + strand, within GFF4603 at 393.397 kb on + strand, within GFF4603 at 393.397 kb on + strand, within GFF4603 at 393.398 kb on - strand, within GFF4603 at 393.398 kb on - strand, within GFF4603 at 393.398 kb on - strand, within GFF4603 at 393.476 kb on + strand, within GFF4603 at 393.477 kb on - strand, within GFF4603 at 393.494 kb on + strand, within GFF4603 at 393.497 kb on - strand, within GFF4603 at 393.589 kb on + strand, within GFF4603 at 393.656 kb on + strand, within GFF4603 at 393.658 kb on + strand, within GFF4603 at 393.659 kb on - strand, within GFF4603 at 393.738 kb on - strand, within GFF4603 at 393.800 kb on + strand at 393.872 kb on + strand at 393.873 kb on - strand at 393.920 kb on + strand at 394.018 kb on - strand at 394.122 kb on - strand at 394.122 kb on - strand at 394.219 kb on - strand, within GFF4604 at 394.219 kb on - strand, within GFF4604 at 394.224 kb on + strand, within GFF4604 at 394.224 kb on + strand, within GFF4604 at 394.225 kb on - strand, within GFF4604 at 394.225 kb on - strand, within GFF4604 at 394.225 kb on - strand, within GFF4604 at 394.246 kb on + strand, within GFF4604 at 394.315 kb on - strand, within GFF4604 at 394.351 kb on + strand, within GFF4604 at 394.394 kb on + strand, within GFF4604 at 394.466 kb on + strand, within GFF4604 at 394.865 kb on - strand, within GFF4604
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 391,634 + GFF4601 0.81 +2.9 392,137 + GFF4602 0.16 -0.2 392,137 + GFF4602 0.16 +0.5 392,138 - GFF4602 0.16 -1.1 392,138 - GFF4602 0.16 -0.1 392,266 - GFF4602 0.41 +0.7 392,270 - GFF4602 0.42 -0.2 392,270 - GFF4602 0.42 -0.3 392,271 + GFF4602 0.42 -0.8 392,294 + GFF4602 0.47 +0.4 392,295 - GFF4602 0.47 +0.1 392,295 - GFF4602 0.47 -0.8 392,295 - GFF4602 0.47 +0.1 392,298 - GFF4602 0.48 -0.3 392,346 - GFF4602 0.57 +0.0 392,513 - +0.1 392,581 - -0.6 392,654 - -0.0 392,656 - -0.1 392,732 + +0.1 392,734 + -0.3 392,734 + -0.0 392,735 - -0.1 392,836 + GFF4603 0.17 -1.1 392,843 + GFF4603 0.18 -2.0 392,843 + GFF4603 0.18 +0.6 392,844 - GFF4603 0.18 -0.3 393,215 - GFF4603 0.46 -0.1 393,215 - GFF4603 0.46 -0.3 393,327 + GFF4603 0.55 -0.4 393,393 + GFF4603 0.60 -0.8 393,397 + GFF4603 0.60 +1.2 393,397 + GFF4603 0.60 -0.3 393,397 + GFF4603 0.60 +0.7 393,398 - GFF4603 0.60 -0.1 393,398 - GFF4603 0.60 +0.1 393,398 - GFF4603 0.60 -0.6 393,476 + GFF4603 0.66 +0.0 393,477 - GFF4603 0.66 -0.2 393,494 + GFF4603 0.67 -0.4 393,497 - GFF4603 0.67 -0.6 393,589 + GFF4603 0.74 -0.3 393,656 + GFF4603 0.79 -0.8 393,658 + GFF4603 0.80 -0.1 393,659 - GFF4603 0.80 -0.5 393,738 - GFF4603 0.86 -0.3 393,800 + -3.1 393,872 + -1.4 393,873 - +0.1 393,920 + -1.0 394,018 - +0.0 394,122 - +0.5 394,122 - -0.2 394,219 - GFF4604 0.13 -0.1 394,219 - GFF4604 0.13 -0.6 394,224 + GFF4604 0.14 +0.0 394,224 + GFF4604 0.14 +0.5 394,225 - GFF4604 0.14 +0.2 394,225 - GFF4604 0.14 -0.2 394,225 - GFF4604 0.14 -0.8 394,246 + GFF4604 0.15 +0.5 394,315 - GFF4604 0.18 -0.5 394,351 + GFF4604 0.19 -1.8 394,394 + GFF4604 0.21 -0.2 394,466 + GFF4604 0.25 +0.4 394,865 - GFF4604 0.43 -0.5
Or see this region's nucleotide sequence