Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4499 and GFF4500 are separated by 308 nucleotides GFF4500 and GFF4501 are separated by 9 nucleotides
GFF4499 - Ferric enterobactin-binding periplasmic protein FepB (TC 3.A.1.14.2), at 285,204 to 286,160
GFF4499
GFF4500 - Isochorismate synthase (EC 5.4.4.2) of siderophore biosynthesis, at 286,469 to 287,644
GFF4500
GFF4501 - 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [enterobactin] siderophore, at 287,654 to 289,264
GFF4501
Position (kb) 286
287
288 Strain fitness (log2 ratio) -1
0
1 at 286.164 kb on - strand at 286.231 kb on - strand at 286.241 kb on - strand at 286.280 kb on - strand at 286.314 kb on + strand at 286.376 kb on - strand at 286.379 kb on + strand at 286.380 kb on - strand at 286.380 kb on - strand at 286.380 kb on - strand at 286.380 kb on - strand at 286.441 kb on - strand at 286.441 kb on - strand at 286.442 kb on + strand at 286.442 kb on + strand at 286.443 kb on - strand at 286.444 kb on + strand at 286.444 kb on + strand at 286.444 kb on + strand at 286.445 kb on - strand at 286.445 kb on - strand at 286.452 kb on + strand at 286.453 kb on - strand at 286.474 kb on + strand at 286.475 kb on - strand at 286.685 kb on - strand, within GFF4500 at 286.929 kb on + strand, within GFF4500 at 286.929 kb on + strand, within GFF4500 at 286.933 kb on + strand, within GFF4500 at 287.254 kb on - strand, within GFF4500 at 287.642 kb on + strand at 287.661 kb on + strand at 287.661 kb on + strand at 287.715 kb on - strand at 287.847 kb on + strand, within GFF4501 at 287.912 kb on + strand, within GFF4501 at 287.912 kb on + strand, within GFF4501 at 287.912 kb on + strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.946 kb on + strand, within GFF4501 at 287.947 kb on - strand, within GFF4501 at 287.990 kb on - strand, within GFF4501 at 288.043 kb on + strand, within GFF4501 at 288.043 kb on + strand, within GFF4501 at 288.043 kb on + strand, within GFF4501 at 288.044 kb on - strand, within GFF4501 at 288.044 kb on - strand, within GFF4501 at 288.229 kb on + strand, within GFF4501 at 288.278 kb on + strand, within GFF4501 at 288.356 kb on - strand, within GFF4501 at 288.359 kb on + strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.514 kb on - strand, within GFF4501
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 286,164 - -0.3 286,231 - -0.0 286,241 - +0.3 286,280 - -0.0 286,314 + +0.3 286,376 - -0.3 286,379 + -0.4 286,380 - +0.2 286,380 - +0.8 286,380 - -0.8 286,380 - -0.1 286,441 - -0.2 286,441 - +0.4 286,442 + -0.5 286,442 + -0.2 286,443 - +0.1 286,444 + -0.0 286,444 + -0.4 286,444 + +0.3 286,445 - +0.2 286,445 - -0.3 286,452 + -0.5 286,453 - -0.8 286,474 + +0.8 286,475 - +0.3 286,685 - GFF4500 0.18 -1.0 286,929 + GFF4500 0.39 -1.2 286,929 + GFF4500 0.39 +0.4 286,933 + GFF4500 0.39 -1.0 287,254 - GFF4500 0.67 -0.5 287,642 + -0.2 287,661 + -0.8 287,661 + +0.6 287,715 - -1.1 287,847 + GFF4501 0.12 +0.3 287,912 + GFF4501 0.16 +0.6 287,912 + GFF4501 0.16 +0.2 287,912 + GFF4501 0.16 +0.2 287,913 - GFF4501 0.16 +0.2 287,913 - GFF4501 0.16 +0.4 287,913 - GFF4501 0.16 +0.8 287,913 - GFF4501 0.16 -0.0 287,946 + GFF4501 0.18 +0.4 287,947 - GFF4501 0.18 -0.3 287,990 - GFF4501 0.21 -0.1 288,043 + GFF4501 0.24 +0.1 288,043 + GFF4501 0.24 -0.8 288,043 + GFF4501 0.24 -0.3 288,044 - GFF4501 0.24 +0.7 288,044 - GFF4501 0.24 -0.4 288,229 + GFF4501 0.36 +1.1 288,278 + GFF4501 0.39 +0.0 288,356 - GFF4501 0.44 +0.4 288,359 + GFF4501 0.44 -0.2 288,360 - GFF4501 0.44 -0.3 288,360 - GFF4501 0.44 -0.2 288,514 - GFF4501 0.53 -0.1
Or see this region's nucleotide sequence