Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4133 and GFF4134 are separated by 23 nucleotides GFF4134 and GFF4135 are separated by 73 nucleotides GFF4135 and GFF4136 are separated by 294 nucleotides
GFF4133 - 6-phospho-3-hexuloisomerase, at 16,279 to 16,845
GFF4133
GFF4134 - D-arabino-3-hexulose 6-phosphate formaldehyde lyase, at 16,869 to 17,504
GFF4134
GFF4135 - FIG01047465: hypothetical protein, at 17,578 to 18,780
GFF4135
GFF4136 - Oxidoreductase, Gfo/Idh/MocA family, at 19,075 to 20,088
GFF4136
Position (kb)
17
18
19 Strain fitness (log2 ratio)
-1
0
1 at 16.676 kb on - strand, within GFF4133 at 16.717 kb on - strand, within GFF4133 at 16.802 kb on - strand at 16.843 kb on + strand at 17.107 kb on - strand, within GFF4134 at 17.119 kb on + strand, within GFF4134 at 17.196 kb on - strand, within GFF4134 at 17.255 kb on + strand, within GFF4134 at 17.255 kb on + strand, within GFF4134 at 17.255 kb on + strand, within GFF4134 at 17.267 kb on - strand, within GFF4134 at 17.394 kb on + strand, within GFF4134 at 17.416 kb on + strand, within GFF4134 at 17.417 kb on - strand, within GFF4134 at 17.579 kb on + strand at 17.580 kb on - strand at 17.580 kb on - strand at 17.613 kb on - strand at 17.669 kb on - strand at 17.702 kb on - strand, within GFF4135 at 17.870 kb on + strand, within GFF4135 at 18.081 kb on + strand, within GFF4135 at 18.901 kb on - strand at 18.977 kb on + strand at 19.016 kb on + strand at 19.256 kb on + strand, within GFF4136 at 19.257 kb on - strand, within GFF4136 at 19.328 kb on + strand, within GFF4136 at 19.328 kb on + strand, within GFF4136 at 19.328 kb on + strand, within GFF4136 at 19.329 kb on - strand, within GFF4136 at 19.329 kb on - strand, within GFF4136 at 19.642 kb on - strand, within GFF4136 at 19.700 kb on - strand, within GFF4136
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 16,676 - GFF4133 0.70 +0.2 16,717 - GFF4133 0.77 +0.6 16,802 - +0.8 16,843 + +0.5 17,107 - GFF4134 0.37 +0.6 17,119 + GFF4134 0.39 +0.1 17,196 - GFF4134 0.51 +1.1 17,255 + GFF4134 0.61 +0.1 17,255 + GFF4134 0.61 -0.6 17,255 + GFF4134 0.61 +0.4 17,267 - GFF4134 0.63 -0.1 17,394 + GFF4134 0.83 -0.9 17,416 + GFF4134 0.86 +0.3 17,417 - GFF4134 0.86 +0.7 17,579 + -0.4 17,580 - +0.6 17,580 - +1.0 17,613 - +0.5 17,669 - +0.5 17,702 - GFF4135 0.10 -0.2 17,870 + GFF4135 0.24 +0.2 18,081 + GFF4135 0.42 +0.4 18,901 - -0.4 18,977 + +0.1 19,016 + -0.2 19,256 + GFF4136 0.18 -0.3 19,257 - GFF4136 0.18 +0.4 19,328 + GFF4136 0.25 -0.0 19,328 + GFF4136 0.25 +0.3 19,328 + GFF4136 0.25 +0.4 19,329 - GFF4136 0.25 +0.3 19,329 - GFF4136 0.25 -0.7 19,642 - GFF4136 0.56 +1.7 19,700 - GFF4136 0.62 -0.0
Or see this region's nucleotide sequence