Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3925 and GFF3926 are separated by 244 nucleotides GFF3926 and GFF3927 are separated by 33 nucleotides GFF3927 and GFF3928 are separated by 93 nucleotides
GFF3925 - Putative inner membrane protein, at 209,567 to 210,625
GFF3925
GFF3926 - UPF0265 protein YeeX, at 210,870 to 211,205
GFF3926
GFF3927 - Threonine kinase in B12 biosynthesis, at 211,239 to 212,105
GFF3927
GFF3928 - Propionate kinase, propanediol utilization (EC 2.7.2.1), at 212,199 to 213,413
GFF3928
Position (kb) 210
211
212 Strain fitness (log2 ratio) -1
0
1
2
3 at 209.934 kb on + strand, within GFF3925 at 210.119 kb on + strand, within GFF3925 at 210.119 kb on + strand, within GFF3925 at 210.295 kb on + strand, within GFF3925 at 210.295 kb on + strand, within GFF3925 at 210.296 kb on - strand, within GFF3925 at 210.475 kb on + strand, within GFF3925 at 210.476 kb on - strand, within GFF3925 at 210.558 kb on + strand at 210.693 kb on - strand at 210.712 kb on + strand at 210.815 kb on + strand at 211.104 kb on + strand, within GFF3926 at 211.104 kb on + strand, within GFF3926 at 211.104 kb on + strand, within GFF3926 at 211.112 kb on + strand, within GFF3926 at 211.193 kb on - strand at 211.212 kb on - strand at 211.227 kb on + strand at 211.323 kb on - strand at 211.340 kb on + strand, within GFF3927 at 211.386 kb on - strand, within GFF3927 at 211.406 kb on - strand, within GFF3927 at 211.459 kb on - strand, within GFF3927 at 211.527 kb on - strand, within GFF3927 at 211.717 kb on - strand, within GFF3927 at 211.832 kb on + strand, within GFF3927 at 211.901 kb on + strand, within GFF3927 at 212.002 kb on - strand, within GFF3927 at 212.109 kb on - strand at 212.141 kb on + strand at 212.142 kb on - strand at 212.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 209,934 + GFF3925 0.35 +0.5 210,119 + GFF3925 0.52 +0.5 210,119 + GFF3925 0.52 -0.0 210,295 + GFF3925 0.69 +0.5 210,295 + GFF3925 0.69 -0.7 210,296 - GFF3925 0.69 +0.5 210,475 + GFF3925 0.86 +0.5 210,476 - GFF3925 0.86 +0.6 210,558 + -0.0 210,693 - +0.6 210,712 + +0.9 210,815 + -0.3 211,104 + GFF3926 0.70 +0.5 211,104 + GFF3926 0.70 +0.0 211,104 + GFF3926 0.70 -0.3 211,112 + GFF3926 0.72 -0.3 211,193 - -1.3 211,212 - +3.1 211,227 + +0.2 211,323 - -0.3 211,340 + GFF3927 0.12 +0.2 211,386 - GFF3927 0.17 +1.1 211,406 - GFF3927 0.19 -0.9 211,459 - GFF3927 0.25 +0.3 211,527 - GFF3927 0.33 -0.0 211,717 - GFF3927 0.55 +0.2 211,832 + GFF3927 0.68 -0.5 211,901 + GFF3927 0.76 -0.4 212,002 - GFF3927 0.88 +0.4 212,109 - -0.2 212,141 + +0.3 212,142 - -0.0 212,192 - -0.1
Or see this region's nucleotide sequence