Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3912 and GFF3913 are separated by 102 nucleotides GFF3913 and GFF3914 are separated by 359 nucleotides
GFF3912 - Histidinol dehydrogenase (EC 1.1.1.23), at 192,932 to 194,236
GFF3912
GFF3913 - ATP phosphoribosyltransferase (EC 2.4.2.17), at 194,339 to 195,238
GFF3913
GFF3914 - Nucleoside-diphosphate-sugar epimerases, at 195,598 to 196,422
GFF3914
Position (kb)
194
195
196 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 193.848 kb on + strand, within GFF3912 at 193.848 kb on + strand, within GFF3912 at 193.849 kb on - strand, within GFF3912 at 194.108 kb on + strand at 194.109 kb on - strand at 194.305 kb on - strand at 194.557 kb on - strand, within GFF3913 at 194.677 kb on + strand, within GFF3913 at 194.678 kb on - strand, within GFF3913 at 194.958 kb on - strand, within GFF3913 at 194.996 kb on + strand, within GFF3913 at 195.025 kb on + strand, within GFF3913 at 195.025 kb on + strand, within GFF3913 at 195.386 kb on + strand at 195.525 kb on + strand at 195.525 kb on + strand at 195.565 kb on + strand at 195.565 kb on + strand at 195.566 kb on - strand at 195.566 kb on - strand at 195.569 kb on + strand at 195.569 kb on + strand at 195.570 kb on - strand at 195.570 kb on - strand at 195.720 kb on + strand, within GFF3914 at 195.739 kb on + strand, within GFF3914 at 195.918 kb on + strand, within GFF3914 at 195.919 kb on - strand, within GFF3914 at 196.009 kb on - strand, within GFF3914 at 196.225 kb on + strand, within GFF3914 at 196.226 kb on - strand, within GFF3914 at 196.226 kb on - strand, within GFF3914 at 196.230 kb on + strand, within GFF3914 at 196.230 kb on + strand, within GFF3914 at 196.232 kb on + strand, within GFF3914 at 196.233 kb on - strand, within GFF3914
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 193,848 + GFF3912 0.70 -2.8 193,848 + GFF3912 0.70 -0.1 193,849 - GFF3912 0.70 -0.2 194,108 + +0.0 194,109 - +0.6 194,305 - +0.1 194,557 - GFF3913 0.24 -0.3 194,677 + GFF3913 0.38 -1.1 194,678 - GFF3913 0.38 -1.0 194,958 - GFF3913 0.69 -0.7 194,996 + GFF3913 0.73 +0.9 195,025 + GFF3913 0.76 -0.1 195,025 + GFF3913 0.76 -0.8 195,386 + -0.2 195,525 + +0.7 195,525 + -0.2 195,565 + -0.5 195,565 + +0.9 195,566 - +0.1 195,566 - +0.1 195,569 + +0.3 195,569 + +0.2 195,570 - -0.5 195,570 - +0.4 195,720 + GFF3914 0.15 +0.8 195,739 + GFF3914 0.17 -1.1 195,918 + GFF3914 0.39 -0.5 195,919 - GFF3914 0.39 +0.2 196,009 - GFF3914 0.50 +1.3 196,225 + GFF3914 0.76 -0.3 196,226 - GFF3914 0.76 -0.4 196,226 - GFF3914 0.76 -0.8 196,230 + GFF3914 0.77 +0.5 196,230 + GFF3914 0.77 -0.0 196,232 + GFF3914 0.77 -0.2 196,233 - GFF3914 0.77 -0.2
Or see this region's nucleotide sequence