Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3904 and GFF3905 are separated by 142 nucleotides GFF3905 and GFF3906 are separated by 80 nucleotides GFF3906 and GFF3907 overlap by 7 nucleotides
GFF3904 - UDP-glucose 6-dehydrogenase (EC 1.1.1.22), at 185,729 to 186,895
GFF3904
GFF3905 - regulator of length of O-antigen component of lipopolysaccharide chains, at 187,038 to 188,021
GFF3905
GFF3906 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 188,102 to 188,713
GFF3906
GFF3907 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 188,707 to 189,483
GFF3907
Position (kb) 187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 186.247 kb on - strand, within GFF3904 at 186.336 kb on - strand, within GFF3904 at 186.377 kb on + strand, within GFF3904 at 186.377 kb on + strand, within GFF3904 at 186.377 kb on + strand, within GFF3904 at 186.454 kb on + strand, within GFF3904 at 186.542 kb on - strand, within GFF3904 at 186.732 kb on - strand, within GFF3904 at 186.732 kb on - strand, within GFF3904 at 186.754 kb on + strand, within GFF3904 at 186.763 kb on + strand, within GFF3904 at 186.763 kb on + strand, within GFF3904 at 186.990 kb on + strand at 186.996 kb on + strand at 187.028 kb on + strand at 187.037 kb on + strand at 187.052 kb on + strand at 187.220 kb on + strand, within GFF3905 at 187.254 kb on + strand, within GFF3905 at 187.257 kb on - strand, within GFF3905 at 187.335 kb on - strand, within GFF3905 at 187.335 kb on - strand, within GFF3905 at 187.443 kb on + strand, within GFF3905 at 187.488 kb on + strand, within GFF3905 at 187.544 kb on + strand, within GFF3905 at 187.628 kb on + strand, within GFF3905 at 187.629 kb on - strand, within GFF3905 at 187.872 kb on + strand, within GFF3905 at 187.942 kb on + strand at 188.001 kb on + strand at 188.002 kb on - strand at 188.279 kb on - strand, within GFF3906 at 188.279 kb on - strand, within GFF3906 at 188.279 kb on - strand, within GFF3906 at 188.313 kb on + strand, within GFF3906 at 188.462 kb on - strand, within GFF3906 at 188.605 kb on + strand, within GFF3906 at 188.607 kb on + strand, within GFF3906 at 188.608 kb on - strand, within GFF3906 at 188.621 kb on + strand, within GFF3906
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 186,247 - GFF3904 0.44 -0.6 186,336 - GFF3904 0.52 -0.3 186,377 + GFF3904 0.56 -1.8 186,377 + GFF3904 0.56 -0.5 186,377 + GFF3904 0.56 -0.6 186,454 + GFF3904 0.62 -2.1 186,542 - GFF3904 0.70 +0.6 186,732 - GFF3904 0.86 +0.5 186,732 - GFF3904 0.86 -0.1 186,754 + GFF3904 0.88 +0.7 186,763 + GFF3904 0.89 -0.3 186,763 + GFF3904 0.89 -1.0 186,990 + -0.3 186,996 + +1.4 187,028 + -0.2 187,037 + +4.4 187,052 + +2.1 187,220 + GFF3905 0.18 +4.8 187,254 + GFF3905 0.22 +2.9 187,257 - GFF3905 0.22 +1.0 187,335 - GFF3905 0.30 +2.5 187,335 - GFF3905 0.30 +3.2 187,443 + GFF3905 0.41 +2.9 187,488 + GFF3905 0.46 +3.1 187,544 + GFF3905 0.51 +2.7 187,628 + GFF3905 0.60 +3.2 187,629 - GFF3905 0.60 +2.1 187,872 + GFF3905 0.85 +2.7 187,942 + +2.9 188,001 + +0.3 188,002 - -0.1 188,279 - GFF3906 0.29 +0.6 188,279 - GFF3906 0.29 +0.2 188,279 - GFF3906 0.29 +0.3 188,313 + GFF3906 0.34 +1.2 188,462 - GFF3906 0.59 -0.6 188,605 + GFF3906 0.82 +0.1 188,607 + GFF3906 0.83 -1.3 188,608 - GFF3906 0.83 -2.6 188,621 + GFF3906 0.85 -0.8
Or see this region's nucleotide sequence