Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3900

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3899 and GFF3900 are separated by 108 nucleotidesGFF3900 and GFF3901 overlap by 14 nucleotides GFF3899 - O antigen biosynthesis rhamnosyltransferase rfbN (EC 2.4.1.-), at 178,616 to 179,560 GFF3899 GFF3900 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 179,669 to 181,000 GFF3900 GFF3901 - Phosphomannomutase (EC 5.4.2.8), at 180,987 to 182,420 GFF3901 Position (kb) 179 180 181 182Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 179.418 kb on + strand, within GFF3899at 179.821 kb on + strand, within GFF3900at 180.952 kb on - strandat 181.252 kb on + strand, within GFF3901at 181.633 kb on + strand, within GFF3901at 181.648 kb on + strand, within GFF3901

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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179,418 + GFF3899 0.85 +4.8
179,821 + GFF3900 0.11 +4.7
180,952 - +4.2
181,252 + GFF3901 0.18 +2.2
181,633 + GFF3901 0.45 +3.1
181,648 + GFF3901 0.46 +4.1

Or see this region's nucleotide sequence