Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF385 and GFF386 are separated by 22 nucleotides GFF386 and GFF387 are separated by 149 nucleotides GFF387 and GFF388 are separated by 100 nucleotides
GFF385 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58), at 99,581 to 100,591
GFF385
GFF386 - Lipopolysaccharide core biosynthesis protein RfaY, at 100,614 to 101,312
GFF386
GFF387 - Lipopolysaccharide core biosynthesis protein RfaZ, at 101,462 to 102,271
GFF387
GFF388 - Lipopolysaccharide 1,2-N-acetylglucosaminetransferase (EC 2.4.1.56), at 102,372 to 103,517
GFF388
Position (kb) 101
102
103 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 100.954 kb on - strand, within GFF386 at 100.958 kb on - strand, within GFF386 at 101.056 kb on + strand, within GFF386 at 101.507 kb on - strand at 101.982 kb on - strand, within GFF387 at 102.025 kb on + strand, within GFF387 at 102.028 kb on - strand, within GFF387 at 102.641 kb on + strand, within GFF388 at 102.671 kb on + strand, within GFF388 at 102.671 kb on + strand, within GFF388 at 102.690 kb on - strand, within GFF388 at 102.759 kb on - strand, within GFF388 at 103.029 kb on - strand, within GFF388 at 103.148 kb on + strand, within GFF388 at 103.152 kb on - strand, within GFF388 at 103.196 kb on + strand, within GFF388 at 103.198 kb on + strand, within GFF388
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 100,954 - GFF386 0.49 +0.2 100,958 - GFF386 0.49 +0.1 101,056 + GFF386 0.63 +0.5 101,507 - -3.1 101,982 - GFF387 0.64 -2.4 102,025 + GFF387 0.70 +0.6 102,028 - GFF387 0.70 -0.0 102,641 + GFF388 0.23 -5.3 102,671 + GFF388 0.26 -2.6 102,671 + GFF388 0.26 -2.7 102,690 - GFF388 0.28 -1.8 102,759 - GFF388 0.34 -1.3 103,029 - GFF388 0.57 -5.7 103,148 + GFF388 0.68 -3.3 103,152 - GFF388 0.68 -2.3 103,196 + GFF388 0.72 -5.5 103,198 + GFF388 0.72 -2.1
Or see this region's nucleotide sequence