Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3604 and GFF3605 overlap by 11 nucleotides GFF3605 and GFF3606 are separated by 11 nucleotides GFF3606 and GFF3607 are separated by 13 nucleotides GFF3607 and GFF3608 overlap by 20 nucleotides
GFF3604 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 355,709 to 357,754
GFF3604
GFF3605 - Type III secretion cytoplasmic ATP synthase (EC 3.6.3.14, YscN,SpaL,MxiB,HrcN,EscN), at 357,744 to 359,045
GFF3605
GFF3606 - Type III secretion spans bacterial envelope protein (YscO), at 359,057 to 359,425
GFF3606
GFF3607 - Type III secretion protein (YscP), at 359,439 to 359,780
GFF3607
GFF3608 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 359,761 to 360,729
GFF3608
Position (kb) 357
358
359
360 Strain fitness (log2 ratio) -1
0
1
2 at 356.790 kb on + strand, within GFF3604 at 356.791 kb on - strand, within GFF3604 at 356.939 kb on - strand, within GFF3604 at 357.123 kb on + strand, within GFF3604 at 357.296 kb on - strand, within GFF3604 at 357.374 kb on + strand, within GFF3604 at 357.510 kb on - strand, within GFF3604 at 357.510 kb on - strand, within GFF3604 at 357.532 kb on + strand, within GFF3604 at 357.554 kb on - strand at 357.799 kb on - strand at 357.841 kb on + strand at 357.863 kb on - strand at 357.884 kb on + strand, within GFF3605 at 357.886 kb on + strand, within GFF3605 at 357.944 kb on - strand, within GFF3605 at 357.998 kb on - strand, within GFF3605 at 358.047 kb on - strand, within GFF3605 at 358.179 kb on - strand, within GFF3605 at 358.271 kb on + strand, within GFF3605 at 358.271 kb on + strand, within GFF3605 at 358.512 kb on + strand, within GFF3605 at 358.610 kb on - strand, within GFF3605 at 358.616 kb on + strand, within GFF3605 at 358.640 kb on + strand, within GFF3605 at 358.644 kb on - strand, within GFF3605 at 358.653 kb on + strand, within GFF3605 at 358.653 kb on + strand, within GFF3605 at 358.733 kb on - strand, within GFF3605 at 358.888 kb on + strand, within GFF3605 at 358.891 kb on + strand, within GFF3605 at 358.922 kb on + strand at 358.946 kb on + strand at 359.009 kb on - strand at 359.032 kb on + strand at 359.454 kb on + strand at 359.487 kb on - strand, within GFF3607 at 359.555 kb on + strand, within GFF3607 at 359.611 kb on + strand, within GFF3607 at 359.741 kb on - strand, within GFF3607 at 359.801 kb on + strand at 359.801 kb on + strand at 359.890 kb on + strand, within GFF3608 at 359.907 kb on + strand, within GFF3608 at 359.996 kb on + strand, within GFF3608 at 359.997 kb on - strand, within GFF3608 at 359.999 kb on - strand, within GFF3608
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 356,790 + GFF3604 0.53 -0.1 356,791 - GFF3604 0.53 -1.3 356,939 - GFF3604 0.60 +0.0 357,123 + GFF3604 0.69 -0.3 357,296 - GFF3604 0.78 +0.5 357,374 + GFF3604 0.81 +0.2 357,510 - GFF3604 0.88 +0.5 357,510 - GFF3604 0.88 -1.0 357,532 + GFF3604 0.89 +0.0 357,554 - -0.5 357,799 - +2.2 357,841 + -0.4 357,863 - +0.6 357,884 + GFF3605 0.11 -0.1 357,886 + GFF3605 0.11 -0.8 357,944 - GFF3605 0.15 -0.5 357,998 - GFF3605 0.20 +0.2 358,047 - GFF3605 0.23 -1.1 358,179 - GFF3605 0.33 +0.9 358,271 + GFF3605 0.40 -0.1 358,271 + GFF3605 0.40 -0.1 358,512 + GFF3605 0.59 +0.3 358,610 - GFF3605 0.67 -1.2 358,616 + GFF3605 0.67 +0.3 358,640 + GFF3605 0.69 -0.4 358,644 - GFF3605 0.69 -0.6 358,653 + GFF3605 0.70 -0.4 358,653 + GFF3605 0.70 -0.3 358,733 - GFF3605 0.76 -0.6 358,888 + GFF3605 0.88 -0.2 358,891 + GFF3605 0.88 +0.3 358,922 + -0.2 358,946 + +0.2 359,009 - -0.5 359,032 + +0.4 359,454 + +0.0 359,487 - GFF3607 0.14 +0.7 359,555 + GFF3607 0.34 +0.5 359,611 + GFF3607 0.50 -0.6 359,741 - GFF3607 0.88 -0.8 359,801 + +0.9 359,801 + +0.6 359,890 + GFF3608 0.13 +0.5 359,907 + GFF3608 0.15 -0.2 359,996 + GFF3608 0.24 -0.2 359,997 - GFF3608 0.24 -0.5 359,999 - GFF3608 0.25 -0.8
Or see this region's nucleotide sequence