Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3575 and GFF3576 overlap by 26 nucleotides GFF3576 and GFF3577 are separated by 97 nucleotides GFF3577 and GFF3578 are separated by 95 nucleotides GFF3578 and GFF3579 are separated by 6 nucleotides
GFF3575 - Tetrathionate reductase sensory transduction histidine kinase, at 333,402 to 335,180
GFF3575
GFF3576 - Tetrathionate reductase two-component response regulator, at 335,155 to 335,775
GFF3576
GFF3577 - FIG00637899: hypothetical protein, at 335,873 to 336,085
GFF3577
GFF3578 - COG1683: Uncharacterized conserved protein / FIG143828: Hypothetical protein YbgA, at 336,181 to 337,062
GFF3578
GFF3579 - hypothetical protein, at 337,069 to 337,203
GFF3579
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1 at 334.970 kb on - strand, within GFF3575 at 334.972 kb on + strand, within GFF3575 at 335.134 kb on + strand at 335.185 kb on - strand at 335.300 kb on + strand, within GFF3576 at 335.408 kb on + strand, within GFF3576 at 335.542 kb on - strand, within GFF3576 at 335.543 kb on + strand, within GFF3576 at 335.543 kb on + strand, within GFF3576 at 335.655 kb on - strand, within GFF3576 at 335.765 kb on - strand at 336.049 kb on + strand, within GFF3577 at 336.049 kb on + strand, within GFF3577 at 336.245 kb on - strand at 336.245 kb on - strand at 336.248 kb on + strand at 336.445 kb on - strand, within GFF3578 at 336.671 kb on - strand, within GFF3578 at 336.789 kb on - strand, within GFF3578 at 336.789 kb on - strand, within GFF3578 at 336.837 kb on + strand, within GFF3578 at 337.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 334,970 - GFF3575 0.88 +0.2 334,972 + GFF3575 0.88 +0.8 335,134 + +0.6 335,185 - +1.2 335,300 + GFF3576 0.23 +0.1 335,408 + GFF3576 0.41 -0.8 335,542 - GFF3576 0.62 -0.7 335,543 + GFF3576 0.62 -0.2 335,543 + GFF3576 0.62 -1.8 335,655 - GFF3576 0.81 -0.6 335,765 - -0.0 336,049 + GFF3577 0.83 -1.1 336,049 + GFF3577 0.83 -0.3 336,245 - +0.4 336,245 - -0.3 336,248 + -0.6 336,445 - GFF3578 0.30 +0.4 336,671 - GFF3578 0.56 +0.0 336,789 - GFF3578 0.69 +0.1 336,789 - GFF3578 0.69 +0.0 336,837 + GFF3578 0.74 +0.0 337,069 - -0.0
Or see this region's nucleotide sequence