Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3413 and GFF3414 are separated by 80 nucleotides GFF3414 and GFF3415 overlap by 1 nucleotides GFF3415 and GFF3416 are separated by 123 nucleotides
GFF3413 - FIG002776: hypothetical protein, at 178,076 to 179,197
GFF3413
GFF3414 - Sensor protein PhoQ (EC 2.7.13.3), at 179,278 to 180,741
GFF3414
GFF3415 - Transcriptional regulatory protein PhoP, at 180,741 to 181,415
GFF3415
GFF3416 - Adenylosuccinate lyase (EC 4.3.2.2), at 181,539 to 182,909
GFF3416
Position (kb) 179
180
181 Strain fitness (log2 ratio) -2
-1
0
1 at 178.289 kb on + strand, within GFF3413 at 178.441 kb on + strand, within GFF3413 at 178.441 kb on + strand, within GFF3413 at 178.442 kb on - strand, within GFF3413 at 178.454 kb on - strand, within GFF3413 at 178.515 kb on + strand, within GFF3413 at 178.518 kb on + strand, within GFF3413 at 178.519 kb on - strand, within GFF3413 at 178.519 kb on - strand, within GFF3413 at 178.523 kb on - strand, within GFF3413 at 178.523 kb on - strand, within GFF3413 at 178.629 kb on + strand, within GFF3413 at 178.799 kb on - strand, within GFF3413 at 178.802 kb on - strand, within GFF3413 at 178.877 kb on - strand, within GFF3413 at 179.126 kb on + strand at 179.184 kb on - strand at 179.191 kb on - strand at 179.218 kb on + strand at 179.218 kb on + strand at 179.373 kb on - strand at 179.425 kb on + strand, within GFF3414 at 179.582 kb on - strand, within GFF3414 at 179.675 kb on + strand, within GFF3414 at 179.741 kb on - strand, within GFF3414 at 179.877 kb on + strand, within GFF3414 at 180.128 kb on - strand, within GFF3414 at 180.153 kb on - strand, within GFF3414 at 180.153 kb on - strand, within GFF3414 at 180.174 kb on + strand, within GFF3414 at 180.177 kb on - strand, within GFF3414 at 180.255 kb on + strand, within GFF3414 at 180.305 kb on - strand, within GFF3414 at 180.365 kb on - strand, within GFF3414 at 180.561 kb on + strand, within GFF3414 at 180.736 kb on - strand at 180.849 kb on - strand, within GFF3415 at 180.877 kb on + strand, within GFF3415 at 181.079 kb on + strand, within GFF3415 at 181.257 kb on + strand, within GFF3415 at 181.298 kb on - strand, within GFF3415 at 181.405 kb on - strand at 181.483 kb on + strand at 181.541 kb on - strand at 181.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 178,289 + GFF3413 0.19 -0.2 178,441 + GFF3413 0.33 -0.5 178,441 + GFF3413 0.33 -0.7 178,442 - GFF3413 0.33 -0.5 178,454 - GFF3413 0.34 +0.5 178,515 + GFF3413 0.39 -0.0 178,518 + GFF3413 0.39 +1.4 178,519 - GFF3413 0.39 -0.4 178,519 - GFF3413 0.39 -0.3 178,523 - GFF3413 0.40 -0.1 178,523 - GFF3413 0.40 -0.4 178,629 + GFF3413 0.49 -1.9 178,799 - GFF3413 0.64 -0.3 178,802 - GFF3413 0.65 -0.9 178,877 - GFF3413 0.71 +0.7 179,126 + -0.9 179,184 - -0.7 179,191 - -0.3 179,218 + -0.7 179,218 + -1.3 179,373 - +0.7 179,425 + GFF3414 0.10 +1.4 179,582 - GFF3414 0.21 +1.0 179,675 + GFF3414 0.27 -2.2 179,741 - GFF3414 0.32 +0.9 179,877 + GFF3414 0.41 -0.2 180,128 - GFF3414 0.58 +0.3 180,153 - GFF3414 0.60 -0.2 180,153 - GFF3414 0.60 +0.3 180,174 + GFF3414 0.61 +0.4 180,177 - GFF3414 0.61 +0.1 180,255 + GFF3414 0.67 +0.4 180,305 - GFF3414 0.70 -0.5 180,365 - GFF3414 0.74 +0.1 180,561 + GFF3414 0.88 +0.4 180,736 - -0.4 180,849 - GFF3415 0.16 +0.7 180,877 + GFF3415 0.20 +1.1 181,079 + GFF3415 0.50 +0.2 181,257 + GFF3415 0.76 +0.3 181,298 - GFF3415 0.83 +1.0 181,405 - +0.1 181,483 + +0.1 181,541 - -0.5 181,545 - +0.7
Or see this region's nucleotide sequence