Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3245 and GFF3246 are separated by 68 nucleotides GFF3246 and GFF3247 are separated by 185 nucleotides GFF3247 and GFF3248 are separated by 71 nucleotides
GFF3245 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59), at 19,774 to 20,292
GFF3245
GFF3246 - ATP-dependent protease La (EC 3.4.21.53) Type II, at 20,361 to 22,121
GFF3246
GFF3247 - Macrodomain Ter protein YcbG, at 22,307 to 22,759
GFF3247
GFF3248 - Outer membrane protein A precursor, at 22,831 to 23,907
GFF3248
Position (kb)
20
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 19.368 kb on + strand at 19.430 kb on + strand at 19.490 kb on + strand at 19.555 kb on + strand at 19.761 kb on - strand at 20.331 kb on - strand at 20.372 kb on + strand at 20.791 kb on - strand, within GFF3246 at 20.791 kb on - strand, within GFF3246 at 20.848 kb on - strand, within GFF3246 at 20.925 kb on - strand, within GFF3246 at 21.288 kb on - strand, within GFF3246 at 21.393 kb on - strand, within GFF3246 at 21.393 kb on - strand, within GFF3246 at 21.830 kb on + strand, within GFF3246 at 21.938 kb on + strand, within GFF3246 at 21.972 kb on + strand at 21.973 kb on - strand at 22.020 kb on + strand at 22.021 kb on - strand at 22.060 kb on - strand at 22.069 kb on + strand at 22.145 kb on + strand at 22.166 kb on - strand at 22.397 kb on + strand, within GFF3247 at 22.489 kb on + strand, within GFF3247 at 23.067 kb on - strand, within GFF3248
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 19,368 + -0.5 19,430 + -0.2 19,490 + -0.6 19,555 + +0.5 19,761 - -0.2 20,331 - -1.6 20,372 + +1.7 20,791 - GFF3246 0.24 +1.6 20,791 - GFF3246 0.24 +1.5 20,848 - GFF3246 0.28 +0.1 20,925 - GFF3246 0.32 -0.6 21,288 - GFF3246 0.53 +0.5 21,393 - GFF3246 0.59 -0.6 21,393 - GFF3246 0.59 -1.4 21,830 + GFF3246 0.83 -1.3 21,938 + GFF3246 0.90 -0.8 21,972 + -1.4 21,973 - +0.5 22,020 + +0.3 22,021 - -0.2 22,060 - +0.1 22,069 + -0.4 22,145 + -2.0 22,166 - -1.2 22,397 + GFF3247 0.20 +2.6 22,489 + GFF3247 0.40 -0.7 23,067 - GFF3248 0.22 +1.5
Or see this region's nucleotide sequence