Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3051 and GFF3052 are separated by 46 nucleotides GFF3052 and GFF3053 are separated by 131 nucleotides GFF3053 and GFF3054 are separated by 17 nucleotides GFF3054 and GFF3055 are separated by 18 nucleotides
GFF3051 - Putative Holliday junction resolvase YggF, at 63,800 to 64,216
GFF3051
GFF3052 - Transcriptional regulator, IclR family, at 64,263 to 64,949
GFF3052
GFF3053 - Twitching motility protein PilT, at 65,081 to 66,061
GFF3053
GFF3054 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 66,079 to 66,783
GFF3054
GFF3055 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 66,802 to 67,368
GFF3055
Position (kb) 65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 64.214 kb on + strand at 64.215 kb on - strand at 64.302 kb on + strand at 64.388 kb on + strand, within GFF3052 at 64.419 kb on + strand, within GFF3052 at 64.602 kb on - strand, within GFF3052 at 64.610 kb on - strand, within GFF3052 at 64.613 kb on + strand, within GFF3052 at 64.613 kb on + strand, within GFF3052 at 64.615 kb on + strand, within GFF3052 at 64.616 kb on - strand, within GFF3052 at 64.619 kb on + strand, within GFF3052 at 64.620 kb on - strand, within GFF3052 at 64.636 kb on - strand, within GFF3052 at 64.636 kb on - strand, within GFF3052 at 64.644 kb on + strand, within GFF3052 at 64.763 kb on - strand, within GFF3052 at 64.835 kb on + strand, within GFF3052 at 64.836 kb on - strand, within GFF3052 at 64.838 kb on + strand, within GFF3052 at 64.839 kb on - strand, within GFF3052 at 64.842 kb on - strand, within GFF3052 at 64.842 kb on - strand, within GFF3052 at 64.842 kb on - strand, within GFF3052 at 64.897 kb on + strand at 64.898 kb on - strand at 64.920 kb on + strand at 64.953 kb on + strand at 65.055 kb on - strand at 65.226 kb on - strand, within GFF3053 at 65.280 kb on - strand, within GFF3053 at 65.345 kb on - strand, within GFF3053 at 65.574 kb on + strand, within GFF3053 at 65.581 kb on - strand, within GFF3053 at 65.587 kb on + strand, within GFF3053 at 65.630 kb on - strand, within GFF3053 at 65.726 kb on + strand, within GFF3053 at 66.022 kb on + strand at 66.022 kb on + strand at 66.055 kb on + strand at 66.055 kb on + strand at 66.056 kb on - strand at 66.061 kb on + strand at 66.063 kb on + strand at 66.063 kb on + strand at 66.106 kb on + strand at 66.166 kb on - strand, within GFF3054 at 66.250 kb on + strand, within GFF3054 at 66.381 kb on + strand, within GFF3054 at 66.382 kb on - strand, within GFF3054 at 66.446 kb on + strand, within GFF3054 at 66.447 kb on - strand, within GFF3054 at 66.596 kb on - strand, within GFF3054 at 66.599 kb on + strand, within GFF3054 at 66.632 kb on + strand, within GFF3054 at 66.677 kb on + strand, within GFF3054 at 66.678 kb on - strand, within GFF3054 at 66.687 kb on + strand, within GFF3054 at 66.723 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.846 kb on + strand at 66.857 kb on + strand at 67.017 kb on + strand, within GFF3055
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 64,214 + -0.1 64,215 - +0.4 64,302 + -0.1 64,388 + GFF3052 0.18 +0.1 64,419 + GFF3052 0.23 -0.4 64,602 - GFF3052 0.49 -0.4 64,610 - GFF3052 0.51 +0.1 64,613 + GFF3052 0.51 -0.2 64,613 + GFF3052 0.51 -0.2 64,615 + GFF3052 0.51 -0.8 64,616 - GFF3052 0.51 -0.6 64,619 + GFF3052 0.52 +0.0 64,620 - GFF3052 0.52 +0.4 64,636 - GFF3052 0.54 -0.3 64,636 - GFF3052 0.54 -0.2 64,644 + GFF3052 0.55 +0.4 64,763 - GFF3052 0.73 -0.4 64,835 + GFF3052 0.83 -0.6 64,836 - GFF3052 0.83 -0.1 64,838 + GFF3052 0.84 -0.3 64,839 - GFF3052 0.84 -0.7 64,842 - GFF3052 0.84 -0.2 64,842 - GFF3052 0.84 +0.0 64,842 - GFF3052 0.84 -0.3 64,897 + +0.9 64,898 - -0.4 64,920 + -0.3 64,953 + -0.1 65,055 - +0.4 65,226 - GFF3053 0.15 -0.6 65,280 - GFF3053 0.20 +0.3 65,345 - GFF3053 0.27 -0.0 65,574 + GFF3053 0.50 -0.4 65,581 - GFF3053 0.51 -0.6 65,587 + GFF3053 0.52 -1.2 65,630 - GFF3053 0.56 -0.4 65,726 + GFF3053 0.66 -0.6 66,022 + -0.3 66,022 + +0.0 66,055 + +0.4 66,055 + -0.2 66,056 - -0.4 66,061 + -0.2 66,063 + +1.0 66,063 + -0.1 66,106 + -0.5 66,166 - GFF3054 0.12 -0.8 66,250 + GFF3054 0.24 +0.4 66,381 + GFF3054 0.43 +0.6 66,382 - GFF3054 0.43 -0.4 66,446 + GFF3054 0.52 -0.4 66,447 - GFF3054 0.52 +0.1 66,596 - GFF3054 0.73 +1.0 66,599 + GFF3054 0.74 -0.3 66,632 + GFF3054 0.78 +0.0 66,677 + GFF3054 0.85 +0.1 66,678 - GFF3054 0.85 +0.3 66,687 + GFF3054 0.86 -0.1 66,723 + -0.3 66,844 + +0.9 66,844 + -3.4 66,844 + -0.6 66,846 + -0.6 66,857 + -2.5 67,017 + GFF3055 0.38 -0.4
Or see this region's nucleotide sequence