Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2887

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2886 and GFF2887 overlap by 4 nucleotidesGFF2887 and GFF2888 are separated by 100 nucleotides GFF2886 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 9,511 to 10,773 GFF2886 GFF2887 - UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14), at 10,770 to 11,855 GFF2887 GFF2888 - regulator of length of O-antigen component of lipopolysaccharide chains, at 11,956 to 13,002 GFF2888 Position (kb) 10 11 12Strain fitness (log2 ratio) -1 0 1 2at 9.954 kb on + strand, within GFF2886at 10.226 kb on - strand, within GFF2886at 10.485 kb on - strand, within GFF2886at 10.485 kb on - strand, within GFF2886at 10.674 kb on + strandat 10.749 kb on + strandat 10.835 kb on - strandat 10.892 kb on + strand, within GFF2887at 10.893 kb on - strand, within GFF2887at 10.936 kb on - strand, within GFF2887at 10.940 kb on + strand, within GFF2887at 10.997 kb on + strand, within GFF2887at 10.998 kb on - strand, within GFF2887at 11.083 kb on + strand, within GFF2887at 11.103 kb on + strand, within GFF2887at 11.288 kb on + strand, within GFF2887at 11.332 kb on - strand, within GFF2887at 11.336 kb on + strand, within GFF2887at 11.336 kb on + strand, within GFF2887at 11.336 kb on + strand, within GFF2887at 11.337 kb on - strand, within GFF2887at 11.525 kb on - strand, within GFF2887at 11.553 kb on + strand, within GFF2887at 11.578 kb on + strand, within GFF2887at 11.606 kb on + strand, within GFF2887at 11.721 kb on - strand, within GFF2887at 11.734 kb on + strand, within GFF2887at 11.760 kb on + strandat 11.876 kb on + strandat 11.883 kb on + strandat 11.884 kb on - strandat 11.924 kb on + strandat 12.076 kb on - strand, within GFF2888at 12.132 kb on + strand, within GFF2888at 12.132 kb on + strand, within GFF2888at 12.132 kb on + strand, within GFF2888at 12.216 kb on + strand, within GFF2888at 12.230 kb on - strand, within GFF2888at 12.230 kb on - strand, within GFF2888at 12.288 kb on + strand, within GFF2888at 12.288 kb on + strand, within GFF2888at 12.291 kb on + strand, within GFF2888at 12.325 kb on - strand, within GFF2888at 12.470 kb on + strand, within GFF2888at 12.470 kb on + strand, within GFF2888at 12.566 kb on - strand, within GFF2888at 12.566 kb on - strand, within GFF2888at 12.633 kb on - strand, within GFF2888at 12.636 kb on - strand, within GFF2888at 12.636 kb on - strand, within GFF2888at 12.701 kb on + strand, within GFF2888at 12.701 kb on + strand, within GFF2888at 12.751 kb on - strand, within GFF2888at 12.751 kb on - strand, within GFF2888at 12.754 kb on - strand, within GFF2888at 12.786 kb on - strand, within GFF2888at 12.801 kb on + strand, within GFF2888at 12.801 kb on + strand, within GFF2888at 12.802 kb on - strand, within GFF2888at 12.802 kb on - strand, within GFF2888at 12.802 kb on - strand, within GFF2888at 12.805 kb on + strand, within GFF2888at 12.806 kb on - strand, within GFF2888

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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9,954 + GFF2886 0.35 +1.8
10,226 - GFF2886 0.57 +0.4
10,485 - GFF2886 0.77 +0.2
10,485 - GFF2886 0.77 +0.2
10,674 + -0.3
10,749 + +0.5
10,835 - -0.1
10,892 + GFF2887 0.11 +0.3
10,893 - GFF2887 0.11 -0.5
10,936 - GFF2887 0.15 -0.2
10,940 + GFF2887 0.16 +1.0
10,997 + GFF2887 0.21 +0.9
10,998 - GFF2887 0.21 +0.9
11,083 + GFF2887 0.29 +0.5
11,103 + GFF2887 0.31 +0.2
11,288 + GFF2887 0.48 +0.7
11,332 - GFF2887 0.52 -0.5
11,336 + GFF2887 0.52 +0.3
11,336 + GFF2887 0.52 +0.6
11,336 + GFF2887 0.52 +0.8
11,337 - GFF2887 0.52 +0.7
11,525 - GFF2887 0.70 -0.0
11,553 + GFF2887 0.72 +0.6
11,578 + GFF2887 0.74 +1.8
11,606 + GFF2887 0.77 +0.2
11,721 - GFF2887 0.88 +0.3
11,734 + GFF2887 0.89 +0.4
11,760 + +1.0
11,876 + -0.2
11,883 + +0.5
11,884 - +0.6
11,924 + +0.6
12,076 - GFF2888 0.11 -0.4
12,132 + GFF2888 0.17 +0.4
12,132 + GFF2888 0.17 +0.0
12,132 + GFF2888 0.17 +0.2
12,216 + GFF2888 0.25 -0.3
12,230 - GFF2888 0.26 -0.1
12,230 - GFF2888 0.26 -0.2
12,288 + GFF2888 0.32 -0.3
12,288 + GFF2888 0.32 +1.1
12,291 + GFF2888 0.32 -0.4
12,325 - GFF2888 0.35 -0.5
12,470 + GFF2888 0.49 +0.2
12,470 + GFF2888 0.49 +0.4
12,566 - GFF2888 0.58 +0.1
12,566 - GFF2888 0.58 -0.7
12,633 - GFF2888 0.65 +0.2
12,636 - GFF2888 0.65 -1.1
12,636 - GFF2888 0.65 -0.4
12,701 + GFF2888 0.71 -0.5
12,701 + GFF2888 0.71 +0.2
12,751 - GFF2888 0.76 +0.4
12,751 - GFF2888 0.76 -0.1
12,754 - GFF2888 0.76 -0.0
12,786 - GFF2888 0.79 -0.5
12,801 + GFF2888 0.81 -0.7
12,801 + GFF2888 0.81 +0.3
12,802 - GFF2888 0.81 -0.1
12,802 - GFF2888 0.81 -0.1
12,802 - GFF2888 0.81 +0.5
12,805 + GFF2888 0.81 -0.0
12,806 - GFF2888 0.81 -0.0

Or see this region's nucleotide sequence